Number of the records: 1

Transposons and satellite DNA: on the origin of the major satellite DNA family in the Chenopodium genome

SYSNO ASEP	0533877
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Transposons and satellite DNA: on the origin of the major satellite DNA family in the Chenopodium genome
Author(s)	Belyayev, Alexander (BU-J)_{RID, ORCID} Josefiová, Jiřina (BU-J) Jandová, Michaela (BU-J) Mahelka, Václav (BU-J)_{RID, ORCID} Krak, Karol (BU-J)_{RID, ORCID} Mandák, Bohumil (BU-J)_{RID, ORCID}
Article number	20
Source Title	Mobile DNA. - : BioMed Central - ISSN 1759-8753 Roč. 11, č. 1 (2020), s. 1-10
Number of pages	10 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	CACTA transposons ; satellite DNA ; Chenopodium
Subject RIV	EF - Botanics
OECD category	Plant sciences, botany
R&D Projects	GA20-20286S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	BU-J - RVO:67985939
UT WOS	000545628600001
EID SCOPUS	85087526637
DOI	10.1186/s13100-020-00219-7
Annotation	Extensive and complex links exist between transposable elements (TEs) and satellite DNA (satDNA), which are the two largest fractions of eukaryotic genome. These relationships have a crucial effect on genome structure, function and evolution. Here, we report a novel case of mutual relationships between TEs and satDNA. In the genomes of Chenopodium s. str. species, the deletion derivatives of tnp2 conserved domain of the newly discovered CACTA-like TE Jozin are involved in generating monomers of the most abundant satDNA family of the Chenopodium satellitome. The analysis of the relative positions of satDNA and different TEs utilizing assembled Illumina reads revealed several associations between satDNA arrays and the transposases of putative CACTA-like elements when an 40 bp fragment of tnp2 served as the start monomer of the satDNA array. The high degree of identity of the consensus satDNA monomers of the investigated species and the tnp2 fragment (from 82.1 to 94.9%) provides evidence of the genesis of CficCI-61-40 satDNA family monomers from analogous regions of their respective parental elements. The results were confirmed via molecular genetic methods and Oxford Nanopore sequencing. The discovered phenomenon leads to the continuous replenishment of species genomes with new identical satDNA monomers, which in turn may increase species satellitomes similarity.
Workplace	Institute of Botany
Contact	Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8
Year of Publishing	2021
Electronic address	https://doi.org/10.1186/s13100-020-00219-7

Number of the records: 1