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Photobiochemical changes in Chlorella g120 culture during trophic conversion (metabolic pathway shift) from heterotrophic to phototrophic growth regime

SYSNO ASEP	0533409
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Photobiochemical changes in Chlorella g120 culture during trophic conversion (metabolic pathway shift) from heterotrophic to phototrophic growth regime
Author(s)	Babaei, Azadeh (MBU-M)_ORCID Ranglová, Karolína (MBU-M)_ORCID Malapascua, José R.F. (MBU-M) Torzillo, G. (IT) Shayegan, J. (IR) Silva Benavides, A. M. (CR) Masojídek, Jiří (MBU-M)_{RID, ORCID}
Number of authors	7
Source Title	Journal of Applied Phycology. - : Springer - ISSN 0921-8971 Roč. 32, č. 5 (2020), s. 2807-2818
Number of pages	12 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Chlorella ; Chlorophyll fluorescence ; Heterotrophy ; Photosynthesis ; Respiration ; Trophic conversion
Subject RIV	DA - Hydrology ; Limnology
OECD category	Marine biology, freshwater biology, limnology
R&D Projects	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	MBU-M - RVO:61388971
UT WOS	000539524100001
EID SCOPUS	85086317714
DOI	10.1007/s10811-020-02137-w
Annotation	Physiological and photobiochemical changes and growth in the heterotrophic strainChlorella vulgarisg120 were studied during trophic conversion from heterotrophic to phototrophic growth regime. After the exposure of theChlorellag120 culture to light, it revealed a significant activity of the electron transport (450-700 mu mol e(-) m(-2) s(-1)as measured by chlorophyll fluorescence) and high PSII photochemical yieldF(v)/F(m)between 0.7 and 0.8. Fast fluorescence induction kinetics showed that PSII electron acceptors in the plastoquinone pool remained partly oxidized, indicating no downregulation of PSII electron transport. The data further revealed that high photobiochemical activity is lost in futile (protective) processes of non-photochemical quenching and respiration which indicate that surplus energy is dissipated in these processes. Pigment analysis showed low chlorophyll content 0.35-1.15% as compared with exclusively phototrophic strainChlorella vulgarisR-117. Nevertheless, the carotenoid content in g120 was relatively high 0.20-0.33% of dry weight which resulted in a considerably high ratio of carotenoid/chlorophyll 0.31-0.44. This strain probably does not possess the fully competent photosynthetic apparatus and can only partially adapt to phototrophy. We show that the heterotrophically grown g120 strain can undergo metabolic shift from heterotrophic to phototrophic growth regime. It might be an interesting strain from biotechnological point of view as a source of carotenoids, especially lutein.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2021
Electronic address	https://link.springer.com/article/10.1007/s10811-020-02137-w

Number of the records: 1