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The negative effect of magnetic nanoparticles with ascorbic acid on peritoneal macrophages
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SYSNO ASEP 0532733 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The negative effect of magnetic nanoparticles with ascorbic acid on peritoneal macrophages Author(s) Jiráková, Klára (UEM-P)
Moskvin, Maksym (UMCH-V) RID, ORCID
Machová-Urdzíková, Lucia (UEM-P) ORCID
Rössner ml., Pavel (UEM-P) RID, ORCID
Elzeinová, Fatima (UEM-P)
Chudíčková, Milada (UEM-P) RID, ORCID
Jirák, D. (CZ)
Ziolkowska, N. (CZ)
Horák, Daniel (UMCH-V) RID, ORCID
Kubinová, Šárka (UEM-P) RID, ORCID
Jendelová, Pavla (UEM-P) RID, ORCIDSource Title Neurochemical Research. - : Springer - ISSN 0364-3190
Roč. 45, č. 1 (2020), s. 159-170Number of pages 11 s. Language eng - English Country US - United States Keywords nanoparticles ; macrophages ; oxidative stress ; cytotoxicity Subject RIV FH - Neurology OECD category Civil engineering Subject RIV - cooperation Institute of Macromolecular Chemistry - Macromolecular Chemistry R&D Projects LTAUSA17120 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA16-14631S GA ČR - Czech Science Foundation (CSF) GA17-04918S GA ČR - Czech Science Foundation (CSF) LO1309 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF15_003/0000419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UEM-P - RVO:68378041 ; UMCH-V - RVO:61389013 UT WOS 000511696200014 EID SCOPUS 85064253798 DOI 10.1007/s11064-019-02790-9 Annotation Superparamagnetic iron oxide nanoparticles (SPIOn) are widely used as a contrast agent for cell labeling. Macrophages are the first line of defense of organisms in contact with nanoparticles after their administration. In this study we investigated the effect of silica-coated nanoparticles (gamma-Fe2O3-SiO2) with or without modification by an ascorbic acid (gamma-Fe2O3-SiO2-ASA), which is meant to act as an antioxidative agent on rat peritoneal macrophages. Both types of nanoparticles were phagocytosed by macrophages in large amounts as confirmed by transmission electron microscopy and Prusian blue staining, however they did not substantially affect the viability of exposed cells in monitored intervals. We further explored cytotoxic effects related to oxidative stress, which is frequently documented in cells exposed to nanoparticles. Our analysis of double strand breaks (DSBs) marker gamma H2AX showed an increased number of DSBs in cells treated with nanoparticles. Nanoparticle exposure further revealed only slight changes in the expression of genes involved in oxidative stress response. Lipid peroxidation, another marker of oxidative stress, was not significantly affirmed after nanoparticle exposure. Our data indicate that the effect of both types of nanoparticles on cell viability, or biomolecules such as DNA or lipids, was similar, however the presence of ascorbic acid, either bound to the nanoparticles or added to the cultivation medium, worsened the negative effect of nanoparticles in various tests performed. The attachment of ascorbic acid on the surface of nanoparticles did not have a protective effect against induced cytotoxicity, as expected. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2021 Electronic address https://link.springer.com/article/10.1007/s11064-019-02790-9
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