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Community dynamics and function of algae and bacteria during winter in central European great lakes
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SYSNO ASEP 0532694 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Community dynamics and function of algae and bacteria during winter in central European great lakes Author(s) Bullerjahn, G. S. (US)
McKay, R. M. L. (US)
Bernát, Gábor (MBU-M) ORCID
Prášil, Ondřej (MBU-M) RID, ORCID
Voros, L. (HU)
Palffy, K. (HU)
Tugyi, N. (HU)
Somogyi, B. (HU)Source Title Journal of Great Lakes Research. - : Elsevier - ISSN 0380-1330
Roč. 46, č. 4 (2020), s. 732-740Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords ice ; diatom ; phytoplankton ; assemblages ; erie Subject RIV DA - Hydrology ; Limnology OECD category Marine biology, freshwater biology, limnology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EE2.3.20.0203 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0392 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000556753300005 EID SCOPUS 85069555303 DOI 10.1016/j.jglr.2019.07.002 Annotation Abundant phytoplankton and bacteria were identified by microscopy and high-throughput 16S rRNA tag Illumina sequencing of samples from water- and ice phases collected during winter at two central European Great Lakes, Balaton and Ferto (Neusiedlersee). Bacterial reads at all sites were dominated (>85%) by Bacteroidetes and Proteobacteria. Amongst phototrophs, microscopy and 16S sequencing revealed that both phytoplankton and cyanobacteria were represented, with a median of 1500 cyanobacterial sequence reads amongst 13 samples analyzed. The sequence analysis compared replicate Balaton and Ferto ice and water samples with an outgroup from three Hungarian soda lakes. In particular, both water and ice from Ferto contained high contributions from cyanobacteria. Two percent of total reads identified to the level of family in water at Nab were dominated by a single operational taxonomic unit (OTU) of a cyanobacterium within the Rivulariaceae, which was largely absent from ice. Conversely, ice samples from both lakes yielded an abundant OTU assigned to a Flavobacterium sp. known to be associated with freshwater ice. Principal Coordinates Analysis (PCoA) revealed that the ice communities from all sites were similar to one another, and that the water communities did not cluster together. Fluorescence emission spectra obtained at 77 K confirmed the presence of intact cyanobacteria in Fento water and ice. Photosynthetic characterization of phototrophs resident in water and ice analyzed by assay of acid-stable photosynthetic (HCO3-)-C-14 incorporation showed that communities from both phases were photosynthetically active, thus adding to growing recognition of ice-covered lakes as viable habitat for phototrophs. (C) 2019 International Association for Great lakes Research. Published by Elsevier B.V. All rights reserved. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://www.sciencedirect.com/science/article/abs/pii/S0380133019301182
Number of the records: 1