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Super-Resolution Microscopy Reveals Diversity of Plant Centromere Architecture
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SYSNO ASEP 0532452 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Super-Resolution Microscopy Reveals Diversity of Plant Centromere Architecture Author(s) Schubert, V. (DE)
Neumann, Pavel (BC-A) RID, ORCID
Marques, A. (DE)
Heckmann, S. (DE)
Macas, Jiří (BC-A) RID, ORCID, SAI
Pedrosa-Harand, A. (BR)
Schubert, I. (DE)
Jang, Tae-Soo (BC-A) ORCID
Houben, A. (DE)Number of authors 9 Article number 3488 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 10 (2020)Number of pages 17 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords Luzula ; microtubule ; monocentromere ; Pisum ; Rhynchospora ; structured illumination microscopy Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects GA17-09750S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000539312100084 EID SCOPUS 85085619267 DOI 10.3390/ijms21103488 Annotation Centromeres are essential for proper chromosome segregation to the daughter cells during mitosis and meiosis. Chromosomes of most eukaryotes studied so far have regional centromeres that form primary constrictions on metaphase chromosomes. These monocentric chromosomes vary from point centromeres to so-called ´meta-polycentromeres´, with multiple centromere domains in an extended primary constriction, as identified in Pisum and Lathyrus species. However, in various animal and plant lineages centromeres are distributed along almost the entire chromosome length. Therefore, they are called holocentromeres. In holocentric plants, centromere-specific proteins, at which spindle fibers usually attach, are arranged contiguously (line-like), in clusters along the chromosomes or in bands. Here, we summarize findings of ultrastructural investigations using immunolabeling with centromere-specific antibodies and super-resolution microscopy to demonstrate the structural diversity of plant centromeres. A classification of the different centromere types has been suggested based on the distribution of spindle attachment sites. Based on these findings we discuss the possible evolution and advantages of holocentricity, and potential strategies to segregate holocentric chromosomes correctly. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://www.mdpi.com/1422-0067/21/10/3488/htm
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