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Mitotic Spindle Attachment to the Holocentric Chromosomes of Cuscuta europaea Does Not Correlate With the Distribution of CENH3 Chromatin

SYSNO ASEP	0532441
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Mitotic Spindle Attachment to the Holocentric Chromosomes of Cuscuta europaea Does Not Correlate With the Distribution of CENH3 Chromatin
Author(s)	Oliveira, Ludmila (BC-A)_{RID, ORCID} Neumann, Pavel (BC-A)_{RID, ORCID} Jang, Tae-Soo (BC-A)_ORCID Klemme, Sonja (BC-A) Schubert, J. (DE) Koblížková, Andrea (BC-A) Houben, A. (DE) Macas, Jiří (BC-A)_{RID, ORCID, SAI}
Number of authors	8
Article number	1799
Source Title	Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X Roč. 10, January (2020)
Number of pages	11 s.
Language	eng - English
Country	CH - Switzerland
Keywords	centromere ; kinetochore ; cenh3 ; holocentric chromosomes ; repetitive DNA analysis ; satellite DNA ; Cuscuta
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Genetics and heredity (medical genetics to be 3)
R&D Projects	GA17-09750S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	BC-A - RVO:60077344
UT WOS	000511969000001
EID SCOPUS	85078471387
DOI	10.3389/fpls.2019.01799
Annotation	The centromere is the region on a chromosome where the kinetochore assembles and spindle microtubules attach during mitosis and meiosis. In the vast majority of eukaryotes, the centromere position is determined epigenetically by the presence of the centromere-specific histone H3 variant CENH3. In species with monocentric chromosomes, CENH3 is confined to a single chromosomal region corresponding to the primary constriction on metaphase chromosomes. By contrast, in holocentrics, CENH3 (and thus centromere activity) is distributed along the entire chromosome length. Here, we report a unique pattern of CENH3 distribution in the holocentric plant Cuscuta europaea. This species expressed two major variants of CENH3, both of which were deposited into one to three discrete regions per chromosome, whereas the rest of the chromatin appeared to be devoid of CENH3. The two CENH3 variants fully co-localized, and their immunodetection signals overlapped with the positions of DAPI-positive heterochromatic bands containing the highly amplified satellite repeat CUS-TR24. This CENH3 distribution pattern contrasted with the distribution of the mitotic spindle microtubules, which attached at uniform density along the entire chromosome length. This distribution of spindle attachment sites proves the holocentric nature of C. europaea chromosomes and also suggests that, in this species, CENH3 either lost its function or acts in parallel to an additional CENH3-free mechanism of kinetochore positioning.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2021
Electronic address	https://www.frontiersin.org/articles/10.3389/fpls.2019.01799/full

Number of the records: 1