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Microfluidic device for concentration and SERS-based detection of bacteria in drinking water
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SYSNO ASEP 0532403 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microfluidic device for concentration and SERS-based detection of bacteria in drinking water Author(s) Krafft, B. (DE)
Týčová, Anna (UIACH-O) ORCID
Urban, R. D. (DE)
Dusny, Ch. (DE)
Belder, D. (DE)Number of authors 5 Source Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 42, 1-2 (2021), s. 86-94Number of pages 9 s. Publication form Print - P Language eng - English Country DE - Germany Keywords porous membranes ; microfluidics ; porous membrane ; surface-enhanced Raman spectrometry Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GJ20-14069Y GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 UT WOS 000535060400001 EID SCOPUS 85085517630 DOI 10.1002/elps.202000048 Annotation There is a constant need for the development of easy-to-operate systems for the rapid and unambiguous identification of bacterial pathogens in drinking water without the requirement for time-consuming culture processes. In this study, we present a disposable and low-cost lab-on-a-chip device utilizing a nanoporous membrane, which connects two stacked perpendicular microfluidic channels. Whereas one of the channels supplies the sample, the second one attracts it by potential-driven forces. Surface-enhanced Raman spectrometry (SERS) is employed as a reliable detection method for bacteria identification. To gain the effect of surface enhancement, silver nanoparticles were added to the sample. The pores of the membrane act as a filter trapping the bodies of microorganisms as well as clusters of nanoparticles creating suitable conditions for sensitive SERS detection. Therein, we focused on the construction and characterization of the device performance. To demonstrate the functionality of the microfluidic chip, we analyzed common pathogens (Escherichia coli DH5 alpha and Pseudomonas taiwanensis VLB120) from spiked tap water using the optimized experimental parameters. The obtained results confirmed our system to be promising for the construction of a disposable optical platform for reliable and rapid pathogen detection which couples their electrokinetic concentration on the integrated nanoporous membrane with SERS detection. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2022 Electronic address http://hdl.handle.net/11104/0310907
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