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Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies
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SYSNO ASEP 0532330 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies Author(s) Musílková, Jana (FGU-C) RID, ORCID
Filová, Elena (FGU-C) RID, ORCID
Pala, J. (CZ)
Matějka, Roman (FGU-C) RID, ORCID, SAI
Hadraba, Daniel (FGU-C) RID, ORCID, SAI
Vondrášek, David (FGU-C) ORCID, SAI
Kaplan, Ondřej (FGU-C) RID
Riedel, Tomáš (UMCH-V) RID, ORCID
Brynda, Eduard (UMCH-V) RID
Kučerová, Johanka (UMCH-V)
Koňařík, M. (CZ)
Lopot, F. (CZ)
Pirk, J. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDArticle number 015008 Source Title Biomedical Materials. - : Institute of Physics Publishing - ISSN 1748-6041
Roč. 15, č. 1 (2020)Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords fibronectin ; human pericardium ; genipin ; glutaraldehyde ; endotelial cells Subject RIV FA - Cardiovascular Diseases incl. Cardiotharic Surgery OECD category Cardiac and Cardiovascular systems Subject RIV - cooperation Institute of Macromolecular Chemistry - Macromolecular Chemistry R&D Projects NV15-29153A GA MZd - Ministry of Health (MZ) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 ; UMCH-V - RVO:61389013 UT WOS 000563778900006 EID SCOPUS 85076327091 DOI 10.1088/1748-605X/ab52db Annotation Decellularized human pericardium is under study as an allogenic material for cardiovascular applications. The effects of crosslinking on the mechanical properties of decellularized pericardium were determined with a uniaxial tensile test, and the effects of crosslinking on the collagen structure of decellularized pericardium were determined by multiphoton microscopy. The viability of human umbilical vein endothelial cells seeded on decellularized human pericardium and on pericardium strongly and weakly crosslinked with glutaraldehyde and with genipin was evaluated by means of an MTS assay. The viability of the cells, measured by their metabolic activity, decreased considerably when the pericardium was crosslinked with glutaraldehyde. Conversely, the cell viability increased when the pericardium was crosslinked with genipin. Coating both non-modified pericardium and crosslinked pericardium with a fibrin mesh or with a mesh containing attached heparin and/or fibronectin led to a significant increase in cell viability. The highest degree of viability was attained for samples that were weakly crosslinked with genipin and modified by means of a fibrin and fibronectin coating. The results indicate a method by which in vivo endothelialization of human cardiac allografts or xenografts could potentially be encouraged. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address https://doi.org/10.1088/1748-605X/ab52db
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