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In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes
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SYSNO ASEP 0531959 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes Author(s) Kashkan, Ivan (UEB-Q) ORCID
Timofeyenko, Ksenia (UEB-Q) ORCID
Kollárová, E. (CZ)
Růžička, Kamil (UEB-Q) ORCIDNumber of authors 4 Article number 868 Source Title Plants. - : MDPI
Roč. 9, č. 7 (2020)Number of pages 10 s. Language eng - English Country CH - Switzerland Keywords dependent auxin gradients ; network ; growth ; cytochrome-p450 ; proteins ; cells ; alternative splicing ; auxin ; cytokinins ; brassinosteroids ; ahp6 ; dwf4 ; pin7 ; D-amino acid oxidase ; genetic screen ; Arabidopsis Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemical research methods R&D Projects EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC18073 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000554288700001 EID SCOPUS 85090747404 DOI 10.3390/plants9070868 Annotation Rapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, using example genes involved in cytokinin signaling, brassinosteroid synthesis and auxin transport, we present a set of reporters devised to visualize their AS events in vivo. These reporters show a differential tissue-specific expression of certain transcripts and reveal that expression of some of the them can be changed by the application of the exogenous hormone. Finally, based on the characterized AS event of the PIN7 auxin efflux carrier, we designed a system that allows a rapid genetic screening for the factors upstream of this AS event. Our innovative toolset can be therefore highly useful for exploring novel regulatory nodes of hormonal pathways and potentially helpful for plant researchers focusing on developmental aspects of AS. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.3390/plants9070868
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