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Analysis of bile acids in human biological samples by microcolumn separation techniques: A review
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SYSNO ASEP 0531799 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of bile acids in human biological samples by microcolumn separation techniques: A review Author(s) Dosedělová, V. (CZ)
Itterheimová, P. (CZ)
Kubáň, Petr (UIACH-O) ORCIDNumber of authors 3 Source Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 42, 1-2 (2021), s. 68-85Number of pages 18 s. Publication form Print - P Language eng - English Country DE - Germany Keywords tandem mass-spectrometry ; quantitative-analysis ; human plasma ; human urine ; clinical analysis ; gc-ms ; hplc-ms ; Microcolumn separation Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 UT WOS 000552288300001 EID SCOPUS 85088459043 DOI 10.1002/elps.202000139 Annotation Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2022 Electronic address http://hdl.handle.net/11104/0310414
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