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The Influence of Negative Pressure and of the Harvesting Site on the Characteristics of Human Adipose Tissue-Derived Stromal Cells from Lipoaspirates

SYSNO ASEP	0531797
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The Influence of Negative Pressure and of the Harvesting Site on the Characteristics of Human Adipose Tissue-Derived Stromal Cells from Lipoaspirates
Author(s)	Trávníčková, Martina (FGU-C)_{RID, ORCID, SAI} Pajorová, Julia (FGU-C) Zárubová, Jana (FGU-C)_{RID, ORCID} Kročilová, Nikola (FGU-C) Molitor, M. (CZ) Bačáková, Lucie (FGU-C)_{RID, ORCID}
Article number	1016231
Source Title	Stem Cells International - ISSN 1687-966X Roč. 2020, Feb 10 (2020)
Number of pages	13 s.
Language	eng - English
Country	US - United States
Keywords	adipose tissue-derived stromal cells ; tissue engineering ; harvesting site ; harvesting site ; proliferation ; viability
Subject RIV	EI - Biotechnology ; Bionics
OECD category	Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
R&D Projects	LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	NV15-33018A GA MZd - Ministry of Health (MZ)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000558988700001
EID SCOPUS	85080861909
DOI	10.1155/2020/1016231
Annotation	Background. Adipose tissue-derived stromal cells (ADSCs) have great potential for cell-based therapies, including tissue engineering. However, various factors can influence the characteristics of isolated ADSCs. Methods. We studied the influence of the harvesting site, i.e., inner thigh (n = 3), outer thigh (n = 7), and abdomen (n = 9), and of negative pressure, i.e., low (-200 mmHg) and high (-700 mmHg), on the characteristics of isolated ADSCs. We counted initial yields of attached cells after isolation. In subsequent passage, we studied the number, viability, diameter, doubling time, mitochondrial activity, and CD surface markers of isolated ADSCs. Results. We revealed higher initial cell yields from the outer thigh region than from the abdomen region. Negative pressure did not influence the cell yields from the outer thigh region, whereas the yields from the abdomen region were higher under high negative pressure than under low negative pressure. In the subsequent passage, in general, no significant relationship was identified between the different negative pressure and ADSC characteristics. No significant difference was observed in the characteristics of thigh ADSCs and abdomen ADSCs. Only on day 1, the diameter was significantly bigger in outer thigh ADSCs than in abdomen ADSCs. Moreover, we noted a tendency of thigh ADSCs (i.e., inner thigh+outer thigh) to reach a higher cell number on day 7. Discussion. The harvesting site and negative pressure can potentially influence initial cell yields from lipoaspirates. However, for subsequent in vitro culturing and for use in tissue engineering, it seems that the harvesting site and the level of negative pressure do not have a crucial or limiting effect on basic ADSC characteristics.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2021
Electronic address	https://www.hindawi.com/journals/sci/2020/1016231/

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