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The Influence of Negative Pressure and of the Harvesting Site on the Characteristics of Human Adipose Tissue-Derived Stromal Cells from Lipoaspirates

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    SYSNO ASEP0531797
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleThe Influence of Negative Pressure and of the Harvesting Site on the Characteristics of Human Adipose Tissue-Derived Stromal Cells from Lipoaspirates
    Author(s) Trávníčková, Martina (FGU-C) RID, ORCID, SAI
    Pajorová, Julia (FGU-C)
    Zárubová, Jana (FGU-C) RID, ORCID
    Kročilová, Nikola (FGU-C)
    Molitor, M. (CZ)
    Bačáková, Lucie (FGU-C) RID, ORCID
    Article number1016231
    Source TitleStem Cells International - ISSN 1687-966X
    Roč. 2020, Feb 10 (2020)
    Number of pages13 s.
    Languageeng - English
    CountryUS - United States
    Keywordsadipose tissue-derived stromal cells ; tissue engineering ; harvesting site ; harvesting site ; proliferation ; viability
    Subject RIVEI - Biotechnology ; Bionics
    OECD categoryTechnologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
    R&D ProjectsLQ1604 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    NV15-33018A GA MZd - Ministry of Health (MZ)
    Method of publishingOpen access
    Institutional supportFGU-C - RVO:67985823
    UT WOS000558988700001
    EID SCOPUS85080861909
    DOI10.1155/2020/1016231
    AnnotationBackground. Adipose tissue-derived stromal cells (ADSCs) have great potential for cell-based therapies, including tissue engineering. However, various factors can influence the characteristics of isolated ADSCs. Methods. We studied the influence of the harvesting site, i.e., inner thigh (n = 3), outer thigh (n = 7), and abdomen (n = 9), and of negative pressure, i.e., low (-200 mmHg) and high (-700 mmHg), on the characteristics of isolated ADSCs. We counted initial yields of attached cells after isolation. In subsequent passage, we studied the number, viability, diameter, doubling time, mitochondrial activity, and CD surface markers of isolated ADSCs. Results. We revealed higher initial cell yields from the outer thigh region than from the abdomen region. Negative pressure did not influence the cell yields from the outer thigh region, whereas the yields from the abdomen region were higher under high negative pressure than under low negative pressure. In the subsequent passage, in general, no significant relationship was identified between the different negative pressure and ADSC characteristics. No significant difference was observed in the characteristics of thigh ADSCs and abdomen ADSCs. Only on day 1, the diameter was significantly bigger in outer thigh ADSCs than in abdomen ADSCs. Moreover, we noted a tendency of thigh ADSCs (i.e., inner thigh+outer thigh) to reach a higher cell number on day 7. Discussion. The harvesting site and negative pressure can potentially influence initial cell yields from lipoaspirates. However, for subsequent in vitro culturing and for use in tissue engineering, it seems that the harvesting site and the level of negative pressure do not have a crucial or limiting effect on basic ADSC characteristics.
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Year of Publishing2021
    Electronic addresshttps://www.hindawi.com/journals/sci/2020/1016231/
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