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Highly synergistic antimicrobial activity of magainin 2 and PGLa peptides is rooted in the formation of supramolecular complexes with lipids
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SYSNO ASEP 0531413 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Highly synergistic antimicrobial activity of magainin 2 and PGLa peptides is rooted in the formation of supramolecular complexes with lipids Author(s) Aisenbrey, Ch. (FR)
Amaro, Mariana (UFCH-W) RID, ORCID
Pospíšil, Petr (UFCH-W)
Hof, Martin (UFCH-W) RID, ORCID
Bechinger, B. (FR)Article number 11652 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords xenopus-laevis ; membranes ; bilayers ; family ; model ; permeability ; enhancement ; mechanism ; diffusion ; topology Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GX19-26854X GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UFCH-W - RVO:61388955 UT WOS 000550031900002 EID SCOPUS 85087930893 DOI 10.1038/s41598-020-68416-1 Annotation Magainin 2 and PGLa are cationic, amphipathic antimicrobial peptides which when added as equimolar mixture exhibit a pronounced synergism in both their antibacterial and pore-forming activities. Here we show for the first time that the peptides assemble into defined supramolecular structures along the membrane interface. The resulting mesophases are quantitatively described by state-of-the art fluorescence self-quenching and correlation spectroscopies. Notably, the synergistic behavior of magainin 2 and PGLa correlates with the formation of hetero-domains and an order-of-magnitude increased membrane affinity of both peptides. Enhanced membrane association of the peptide mixture is only observed in the presence of phophatidylethanolamines but not of phosphatidylcholines, lipids that dominate bacterial and eukaryotic membranes, respectively. Thereby the increased membrane-affinity of the peptide mixtures not only explains their synergistic antimicrobial activity, but at the same time provides a new concept to increase the therapeutic window of combinatorial drugs. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2021 Electronic address http://hdl.handle.net/11104/0310078
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