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Cell type specific adhesion to surfaces functionalised by amine plasma polymers
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SYSNO ASEP 0531289 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cell type specific adhesion to surfaces functionalised by amine plasma polymers Author(s) Černochová, P. (CZ)
Blahová, L. (CZ)
Medalová, J. (CZ)
Nečas, D. (CZ)
Michlíček, M. (CZ)
Kaushik, P. (CZ)
Přibyl, J. (CZ)
Bartošíková, J. (CZ)
Manakhov, A. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCID
Zajíčková, L. (CZ)Article number 9357 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords amine plasma polymers ; fibroblasts ; keratinocytes ; rat vascular smooth muscle cells ; endothelial cells ; cellular attachment ; spontaneous migration Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects GA18-12774S GA ČR - Czech Science Foundation (CSF) Research Infrastructure CIISB - 90043 - Masarykova univerzita
CEITEC Nano - 90041 - Vysoké učení technické v BrněMethod of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000543956100001 EID SCOPUS 85086163488 DOI 10.1038/s41598-020-65889-y Annotation Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address https://www.nature.com/articles/s41598-020-65889-y
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