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The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution
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SYSNO ASEP 0525646 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution Author(s) Ganesh, Sravya (UMG-J)
Horvat, Filip (UMG-J)
Drutovič, Dávid (UZFG-Y) ORCID
Efenberková, Michaela (UMG-J)
Pinkas, Dominik (UMG-J) ORCID
Jindrová, Anna (UZFG-Y) ORCID
Pasulka, Josef (UMG-J)
Iyyappan, Rajan (UZFG-Y) ORCID
Malík, Radek (UMG-J) RID
Šušor, Andrej (UZFG-Y) RID, ORCID
Vlahovicek, K. (HR)
Šolc, Petr (UZFG-Y) RID, ORCID
Svoboda, Petr (UMG-J) RIDNumber of authors 13 Source Title Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 48, č. 6 (2020), s. 3211-3227Number of pages 17 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords gene-expression ; messenger-rna ; mouse oocytes ; maturation ; single ; cell ; normalization ; transcription ; evolution ; sirnas Subject RIV EB - Genetics ; Molecular Biology OECD category Reproductive biology (medical aspects to be 3) Subject RIV - cooperation Institute of Animal Physiology and Genetics - Genetics ; Molecular Biology R&D Projects LO1419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1609 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2011032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015040 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA18-19395S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UMG-J - RVO:68378050 ; UZFG-Y - RVO:67985904 UT WOS 000525957500035 DOI 10.1093/nar/gkz1239 Annotation Tens of thousands of rapidly evolving long non-coding RNA (lncRNA) genes have been identified, but functions were assigned to relatively few of them. The lncRNA contribution to the mouse oocyte physiology remains unknown. We report the evolutionary history and functional analysis of Sirena1, the most expressed lncRNA and the 10th most abundant poly(A) transcript in mouse oocytes. Sirena1 appeared in the common ancestor of mouse and rat and became engaged in two different post-transcriptional regulations. First, antisense oriented Bob pseudogene insertion into Sirena1 exon 1 is a source of small RNAs targeting Sob mRNA via RNA interference. Second, Sirena1 evolved functional cytoplasmic polyadenylation elements, an unexpected feature borrowed from translation control of specific maternal mRNAs. Sirena1 knock-out does not affect fertility, but causes minor dysregulation of the maternal transcriptome. This includes increased levels of Elob and mitochondrial mRNAs. Mitochondria in Sirena1(-/-) oocytes disperse from the perinuclear compartment, but do not change in number or ultrastructure. Taken together, Sirenal contributes to RNA interference and mitochondrial aggregation in mouse oocytes. Sirena1 exemplifies how lncRNAs stochastically engage or even repurpose molecular mechanisms during evolution. Simultaneously, Sirenai1 expression levels and unique functional features contrast with the lack of functional importance assessed under laboratory conditions. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2021 Electronic address https://academic.oup.com/nar/article/48/6/3211/5709715
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