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Combined effect of lasioglossin LL-III derivative with azoles against Candida albicans virulence factors: biofilm formation, phospholipases, proteases and hemolytic activity

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    SYSNO ASEP0524988
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleCombined effect of lasioglossin LL-III derivative with azoles against Candida albicans virulence factors: biofilm formation, phospholipases, proteases and hemolytic activity
    Author(s) Vaňková, Eva (UOCHB-X)
    Kašparová, Petra (UOCHB-X) ORCID
    Dulíčková, Nikola (UOCHB-X)
    Čeřovský, Václav (UOCHB-X) RID, ORCID
    Article numberfoaa020
    Source TitleFEMS Yeast Research - ISSN 1567-1356
    Roč. 20, č. 3 (2020)
    Number of pages16 s.
    Languageeng - English
    CountryGB - United Kingdom
    Keywordsantimicrobial peptides ; azoles ; biofilm formation ; Candida albicans ; LL-III derivative ; virulence factors
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    R&D ProjectsNV16-27726A GA MZd - Ministry of Health (MZ)
    Method of publishingLimited access
    Institutional supportUOCHB-X - RVO:61388963
    UT WOS000536499200008
    EID SCOPUS85085265115
    DOI10.1093/femsyr/foaa020
    AnnotationCandida albicans has several virulence factors at its disposal, including yeast–hyphal transition associated with biofilm formation, phospholipases, proteases and hemolytic activity, all of which contribute to its pathogenesis. We used synthetic derivative LL-III/43 of antimicrobial peptide lasioglossin LL-III to enhance effect of azoles on attenuation of C. albicans virulence factors. LL-III/43 was able to inhibit initial adhesion or biofilm formation of C. albicans strains at 50 µM. Azoles, however, were ineffective at this concentration. Using fluorescently labeled LL-III/43, we observed that peptide covered C. albicans cells, partially penetrated through their membranes and then accumulated inside cells. LL-III/43 (25 µM) in combination with clotrimazole prevented biofilm formation already at 3.1 µM clotrimazole. Neither LL-III/43 nor azoles were able to significantly inhibit phospholipases, proteases, or hemolytic activity of C. albicans. LL-III/43 (25 µM) and clotrimazole (50 µM) in combination decreased production of these virulence factors, and it completely attenuated its hemolytic activity. Scanning electron microscopy showed that LL-III/43 (50 µM) prevented C. albicans biofilm formation on Ti-6Al-4 V alloy used in orthopedic surgeries and combination of LL-III/43 (25 µM) with clotrimazole (3.1 µM) prevented biofilm formation on urinary catheters. Therefore, mixture of LL-III/43 and clotrimazole is suitable candidate for future pharmaceutical research.
    WorkplaceInstitute of Organic Chemistry and Biochemistry
    Contactasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
    Year of Publishing2021
    Electronic addresshttps://academic.oup.com/femsyr/article-abstract/20/3/foaa020/5824167?redirectedFrom=fulltext
Number of the records: 1