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Combined effect of lasioglossin LL-III derivative with azoles against Candida albicans virulence factors: biofilm formation, phospholipases, proteases and hemolytic activity
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SYSNO ASEP 0524988 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Combined effect of lasioglossin LL-III derivative with azoles against Candida albicans virulence factors: biofilm formation, phospholipases, proteases and hemolytic activity Author(s) Vaňková, Eva (UOCHB-X)
Kašparová, Petra (UOCHB-X) ORCID
Dulíčková, Nikola (UOCHB-X)
Čeřovský, Václav (UOCHB-X) RID, ORCIDArticle number foaa020 Source Title FEMS Yeast Research. - : Oxford University Press - ISSN 1567-1356
Roč. 20, č. 3 (2020)Number of pages 16 s. Language eng - English Country GB - United Kingdom Keywords antimicrobial peptides ; azoles ; biofilm formation ; Candida albicans ; LL-III derivative ; virulence factors Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects NV16-27726A GA MZd - Ministry of Health (MZ) Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 UT WOS 000536499200008 EID SCOPUS 85085265115 DOI 10.1093/femsyr/foaa020 Annotation Candida albicans has several virulence factors at its disposal, including yeast–hyphal transition associated with biofilm formation, phospholipases, proteases and hemolytic activity, all of which contribute to its pathogenesis. We used synthetic derivative LL-III/43 of antimicrobial peptide lasioglossin LL-III to enhance effect of azoles on attenuation of C. albicans virulence factors. LL-III/43 was able to inhibit initial adhesion or biofilm formation of C. albicans strains at 50 µM. Azoles, however, were ineffective at this concentration. Using fluorescently labeled LL-III/43, we observed that peptide covered C. albicans cells, partially penetrated through their membranes and then accumulated inside cells. LL-III/43 (25 µM) in combination with clotrimazole prevented biofilm formation already at 3.1 µM clotrimazole. Neither LL-III/43 nor azoles were able to significantly inhibit phospholipases, proteases, or hemolytic activity of C. albicans. LL-III/43 (25 µM) and clotrimazole (50 µM) in combination decreased production of these virulence factors, and it completely attenuated its hemolytic activity. Scanning electron microscopy showed that LL-III/43 (50 µM) prevented C. albicans biofilm formation on Ti-6Al-4 V alloy used in orthopedic surgeries and combination of LL-III/43 (25 µM) with clotrimazole (3.1 µM) prevented biofilm formation on urinary catheters. Therefore, mixture of LL-III/43 and clotrimazole is suitable candidate for future pharmaceutical research. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2021 Electronic address https://academic.oup.com/femsyr/article-abstract/20/3/foaa020/5824167?redirectedFrom=fulltext
Number of the records: 1