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An appropriate method for assessing hydrogel pore sizes by cryo-sem

SYSNO ASEP	0524886
Document Type	C - Proceedings Paper (int. conf.)
R&D Document Type	Conference Paper
Title	An appropriate method for assessing hydrogel pore sizes by cryo-sem
Author(s)	Adámková, Kateřina (UPT-D) Trudicová, M. (CZ) Hrubanová, Kamila (UPT-D)_{RID, SAI, ORCID} Sedláček, P. (CZ) Krzyžánek, Vladislav (UPT-D)_{RID, ORCID, SAI}
Number of authors	5
Source Title	10th Anniversary International Conference on Nanomaterials - Research and Application (NANOCON 2018). - Ostrava : Tanger, 2019 - ISBN 978-80-87294-89-5
Pages	s. 415-420
Number of pages	6 s.
Publication form	Print - P
Action	Anniversary International Conference on Nanomaterials - Research and Application (NANOCON) /10./
Event date	17.10.2018 - 19.10.2018
VEvent location	Brno
Country	CZ - Czech Republic
Event type	EUR
Language	eng - English
Country	CZ - Czech Republic
Keywords	hydrogelcryo-SEM ; agarose ; cryo-SEM ; freezing methods ; image analysis
Subject RIV	JA - Electronics ; Optoelectronics, Electrical Engineering
OECD category	Polymer science
R&D Projects	GA17-15451S GA ČR - Czech Science Foundation (CSF)
	LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UPT-D - RVO:68081731
UT WOS	000513131900072
EID SCOPUS	85062956246
Annotation	The aim of our work was to examine and describe ultrastructure of the agarose hydrogel and any possible structural concentration dependencies, and to assess the distribution and size of pores of agarose hydrogel in dependence on its concentration. Four concentrations were prepared (0.5 %, 1.0 %, 2.0 % and 4.0 % of dry weight content) and cryo-SEM and turbidimetry methods were executed on wet (original) samples in order to image the ultrastructure and measure the pore sizes within. Reasonable results were obtained for the wet samples as they were closer to their native state they are usually used for applications in. Cryo-SEM and turbidimetry provided comparable results of pore diameters and allowed to compare pore diameters dependant on the concentrations, moreover, it showed more detailed and realistic structure.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2021

Number of the records: 1