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Mixed polar-nonpolar lipid films as minimalistic models of Tear Film Lipid Layer: A Langmuir trough and fluorescence microscopy study

SYSNO ASEP	0524872
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Mixed polar-nonpolar lipid films as minimalistic models of Tear Film Lipid Layer: A Langmuir trough and fluorescence microscopy study
Author(s)	Olžyńska, Agnieszka (UFCH-W)_RID Wizert, A. (PL) Štefl, Martin (UFCH-W) Iskander, D. R. (PL) Cwiklik, Lukasz (UFCH-W)_{RID, ORCID}
Article number	183300
Source Title	Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736 Roč. 1862, č. 9 (2020)
Number of pages	10 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Fluorescence microscopy ; Langmuir trough ; Lipid films ; Tear film lipid layer
Subject RIV	CF - Physical ; Theoretical Chemistry
OECD category	Physical chemistry
R&D Projects	GA18-26751S GA ČR - Czech Science Foundation (CSF)
	GJ19-08304Y GA ČR - Czech Science Foundation (CSF)
Method of publishing	Limited access
Institutional support	UFCH-W - RVO:61388955
UT WOS	000540854800006
EID SCOPUS	85083335546
DOI	10.1016/j.bbamem.2020.183300
Annotation	The Tear Film Lipid Layer (TFLL) covering the surface of the aqueous film at human cornea forms a first barrier between the eye and environment. Its alterations are related to dry eye disease. TFLL is formed by a complex mixture of lipids, with an excess of nonpolar components and a minor fraction of polar molecules. Its thickness is up to 160 nm, hence a multilayer-like structure of TFLL is assumed. However, details of TFLL organization are mostly unavailable in vivo due to the dynamic nature of the human tear film. To overcome this issue, we employ a minimalistic in vitro lipid model of TFLL. We study its biophysical characteristics by using a combination of the Langmuir trough with fluorescence microscopy. The model consists of two-component polar-nonpolar lipid films with a varying component ratio spread on the aqueous subphase at physiologically relevant temperature. We demonstrate that the model lipid mixture undergoes substantial structural reorganization as a function of lateral pressure and polar to nonpolar lipid ratio. In particular, the film is one-molecule-thick and homogenous under low lateral pressure. Upon compression, it transforms into a multilayer structure with inhomogeneities in the form of polar-nonpolar lipid assemblies. Based on this model, we hypothesize that TFLL in vivo has a duplex polar-nonpolar structure and it contains numerous mixed lipid aggregates formed because of film restructuring. These findings, despite the simplified character of the model, seem relevant for TFLL physiology as well as for understanding pathological conditions related to the lipids of the tear film.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2021
Electronic address	http://hdl.handle.net/11104/0309118

Number of the records: 1