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Properties of Lipid Models of Lung Surfactant Containing Cholesterol and Oxidized Lipids: A Mixed Experimental and Computational Study
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SYSNO ASEP 0524869 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Properties of Lipid Models of Lung Surfactant Containing Cholesterol and Oxidized Lipids: A Mixed Experimental and Computational Study Author(s) Olžyńska, Agnieszka (UFCH-W) RID
Delcroix, Pauline (UFCH-W)
Dolejsova, T. (CZ)
Krzaczek, K. (PL)
Korchowiec, B. (PL)
Czogalla, A. (PL)
Cwiklik, Lukasz (UFCH-W) RID, ORCIDSource Title Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 36, č. 4 (2020), s. 1023-1033Number of pages 11 s. Language eng - English Country US - United States Keywords pulmonary surfactant ; dynamics ; membranes ; simulations ; monolayers ; calcium Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GA17-06792S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UFCH-W - RVO:61388955 UT WOS 000511509900024 EID SCOPUS 85079021004 DOI 10.1021/acs.langmuir.9b02469 Annotation We introduce and study a multicomponent lipid film mimicking lipid composition of the human lung surfactant. It consists of phospholipids with various lipid headgroups and tail saturation. Furthermore, it includes cholesterol and oxidized lipids. Langmuir trough and fluorescence microscopy experiments are combined with fully atomistic molecular dynamics simulations. The considered lipid mixtures form complex interfacial films with properties modulated by lateral compression. Cholesterol laterally condenses, and oxidized lipids laterally expand the films. Both types of molecules increase film miscibility. Oxidized lipids also alter the lipid-water interface enhancing film hydration. This effect can be partially reversed by cholesterol. Regarding presentation of different chemical moieties toward the aqueous subphase, the zwitterionic phosphatidylcholine groups dominate at the lipid-water interface, while both the negatively charged phosphatidylglycerol and hydroxyl group of cholesterol are less exposed. The investigated synthetic lipid-only mimic of the lung surfactant may serve as a basis for further studies involving nonlipid pulmonary surfactant components. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2021 Electronic address http://hdl.handle.net/11104/0309114
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