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Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein
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SYSNO ASEP 0524528 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein Author(s) Šebest, Peter (BFU-R) ORCID
Fojt, Lukáš (BFU-R) RID, ORCID
Ostatná, Veronika (BFU-R) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCID
Daňhel, Aleš (BFU-R) RID, ORCIDNumber of authors 5 Article number 107436 Source Title Bioelectrochemistry. - : Elsevier - ISSN 1567-5394
Roč. 132, APR 2020 (2020)Number of pages 9 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords voltammetric determination ; label ; electrochemistry ; oligonucleotides ; nanoparticles Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GJ17-23634Y GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000518670600036 EID SCOPUS 85076459677 DOI 10.1016/j.bioelechem.2019.107436 Annotation Catalytic properties and high adsorption affinity of nucleic acids and proteins to silver amalgam electrode surface make this kind of electrified interface perspective for bioanalytical and biomedical applications. For the first time, a basal-plane pyrolytic graphite electrode (bPGE) has been used as a substrate for electrodeposition of silver amalgam particles (AgAPs). Optimization of the resulting composition, surface morphology and electrochemical properties of the AgAPs was done by scanning electron microscopy with energy disperse X-ray spectroscopy, image processing software and voltammetric detection of electrochemically reducible model organic nitro compound, 4-nitrophenol. Spectro-electrochemical applicability of bPGE-AgAP has been demonstrated by electrolysis of 4-nitrophenol. Simultaneous UV-Vis-chronoamperometry provided information on the number of exchange electrons and the reduction rate constants. Preferential adsorption of the fluorescently labelled calf thymus DNA and the green fluorescent protein (GFP) on the surface of AgAPs was observed by fluorescence microscopy. In contrast to previously studied indium-tin oxide and vapour-deposited gold decorated by AgAPs, herein the presented bPGE-AgAP has provided sufficiently wide negative potential window allowing direct electroanalysis of non labelled DNA and GFP using intrinsic electrochemical signals independently of the fluorescent labelling. The bPGE-AgAP can thus be expected to find application opportunities in protein electrochemistry, (bio) sensor development or in-situ spectro-electrochemical studies. (C) 2019 Elsevier B.V. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2021 Electronic address https://www.sciencedirect.com/science/article/pii/S1567539419304098?via%3Dihub
Number of the records: 1