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Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein

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    SYSNO ASEP0524528
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleElectrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein
    Author(s) Šebest, Peter (BFU-R) ORCID
    Fojt, Lukáš (BFU-R) RID, ORCID
    Ostatná, Veronika (BFU-R) RID, ORCID
    Fojta, Miroslav (BFU-R) RID, ORCID
    Daňhel, Aleš (BFU-R) RID, ORCID
    Number of authors5
    Article number107436
    Source TitleBioelectrochemistry. - : Elsevier - ISSN 1567-5394
    Roč. 132, APR 2020 (2020)
    Number of pages9 s.
    Publication formOnline - E
    Languageeng - English
    CountryCH - Switzerland
    Keywordsvoltammetric determination ; label ; electrochemistry ; oligonucleotides ; nanoparticles
    Subject RIVCE - Biochemistry
    OECD categoryBiochemistry and molecular biology
    R&D ProjectsEF15_003/0000477 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    GJ17-23634Y GA ČR - Czech Science Foundation (CSF)
    Method of publishingLimited access
    Institutional supportBFU-R - RVO:68081707
    UT WOS000518670600036
    EID SCOPUS85076459677
    AnnotationCatalytic properties and high adsorption affinity of nucleic acids and proteins to silver amalgam electrode surface make this kind of electrified interface perspective for bioanalytical and biomedical applications. For the first time, a basal-plane pyrolytic graphite electrode (bPGE) has been used as a substrate for electrodeposition of silver amalgam particles (AgAPs). Optimization of the resulting composition, surface morphology and electrochemical properties of the AgAPs was done by scanning electron microscopy with energy disperse X-ray spectroscopy, image processing software and voltammetric detection of electrochemically reducible model organic nitro compound, 4-nitrophenol. Spectro-electrochemical applicability of bPGE-AgAP has been demonstrated by electrolysis of 4-nitrophenol. Simultaneous UV-Vis-chronoamperometry provided information on the number of exchange electrons and the reduction rate constants. Preferential adsorption of the fluorescently labelled calf thymus DNA and the green fluorescent protein (GFP) on the surface of AgAPs was observed by fluorescence microscopy. In contrast to previously studied indium-tin oxide and vapour-deposited gold decorated by AgAPs, herein the presented bPGE-AgAP has provided sufficiently wide negative potential window allowing direct electroanalysis of non labelled DNA and GFP using intrinsic electrochemical signals independently of the fluorescent labelling. The bPGE-AgAP can thus be expected to find application opportunities in protein electrochemistry, (bio) sensor development or in-situ spectro-electrochemical studies. (C) 2019 Elsevier B.V. All rights reserved.
    WorkplaceInstitute of Biophysics
    ContactJana Poláková,, Tel.: 541 517 244
    Year of Publishing2021
    Electronic address
Number of the records: 1