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Catalytic and redox activity of nucleic acids at mercury electrodes: Roles of nucleobase residues

SYSNO ASEP	0524254
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Catalytic and redox activity of nucleic acids at mercury electrodes: Roles of nucleobase residues
Author(s)	Římánková, Ludmila (BFU-R) Hasoň, Stanislav (BFU-R)_{RID, ORCID} Daňhel, Aleš (BFU-R)_{RID, ORCID} Fojta, Miroslav (BFU-R)_{RID, ORCID} Ostatná, Veronika (BFU-R)_{RID, ORCID}
Number of authors	5
Article number	113812
Source Title	Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657 Roč. 858, FEB 1 2020 (2020)
Number of pages	6 s.
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	hydrogen evolution ; dna ; electrochemistry ; oligonucleotides
Subject RIV	CB - Analytical Chemistry, Separation
OECD category	Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
R&D Projects	GA17-08971S GA ČR - Czech Science Foundation (CSF)
	EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	BFU-R - RVO:68081707
UT WOS	000515205600043
EID SCOPUS	85077739525
DOI	10.1016/j.jelechem.2019.113812
Annotation	Using a series of specifically designed oligonucleotides we have identified adenine and cytosine nucleobases as residues involved in catalytic hydrogen evolution reaction (CHER) of nucleic acids at the hanging mercury drop electrode (HMDE). Due to the CHER, nucleic acids yield catalytic peak H-NA allowing their label-free and reagent-less analysis at low concentrations. Additionally, our results suggest that presence of the electroactive bases (adenine and cytosine) facilitates guanine reduction which is for the first time linked to a signal measurable at the HMDE the peak P. We assume that the peak P is connected with reduction of guanine to 7,8-dihydroguanine, of which reoxidation to guanine is detected at the electrode by the earlier described anodic peak G. Processes connected with the above mentioned signals were studied using cyclic voltammetry by inspection of dependences on experimental parameters such as negative vertex potential and pH. (C) 2019 Elsevier B.V. All rights reserved.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2021
Electronic address	https://www.sciencedirect.com/science/article/pii/S157266571931080X?via%3Dihub

Number of the records: 1