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Gradual cold acclimation induces cardioprotection without affecting adrenergic beta-receptor-mediated adenylyl cyclase signaling
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SYSNO ASEP 0524244 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Gradual cold acclimation induces cardioprotection without affecting adrenergic beta-receptor-mediated adenylyl cyclase signaling Author(s) Tibenská, V. (CZ)
Benešová, A. (CZ)
Vebr, P. (CZ)
Liptáková, A. (CZ)
Hejnová, L. (CZ)
Elsnicová, B. (CZ)
Drahota, Zdeněk (FGU-C) RID, ORCID
Horníková, D. (CZ)
Galatík, F. (CZ)
Kolář, D. (CZ)
Vybíral, S. (CZ)
Alánová, Petra (FGU-C) RID, ORCID
Novotný, J. (CZ)
Kolář, František (FGU-C) RID, ORCID, SAI
Nováková, Olga (FGU-C)
Žurmanová, J.M. (CZ)Source Title Journal of Applied Physiology. - : American Physiological Society - ISSN 8750-7587
Roč. 128, č. 4 (2020), s. 1023-1032Number of pages 10 s. Language eng - English Country US - United States Keywords adenylyl cyclase ; beta-adrenergic receptors ; cardioprotection ; cold acclimation ; mitochondria Subject RIV ED - Physiology OECD category Physiology (including cytology) R&D Projects GA17-07748S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000528322200031 EID SCOPUS 85083545249 DOI 10.1152/japplphysiol.00511.2019 Annotation Novel strategies are needed that can stimulate endogenous signaling pathways to protect the heart from myocardial infarction. The present study tested the hypothesis that appropriate regimen of cold acclimation (CA) may provide a promising approach for improving myocardial resistance to ischemia/reperfusion (UR) injury without negative side effects. We evaluated myocardial UR injury, mitochondrial swelling, and beta-adrenergic receptor (beta-AR)-adenylyl cyclase-mediated signaling. Male Wistar rats were exposed to CA (8 degrees C, 8 h/day for a week, followed by 4 wk at 8 degrees C for 24 h/day), while the recovery group (CAR) was kept at 24 degrees C for an additional 2 wk. The myocardial infarction induced by coronary occlusion for 20 min followed by 3-h reperfusion was reduced from 56% in controls to 30% and 23% after CA and CAR. respectively. In line, the rate of mitochondrial swelling at 200 mu M Ca2+ was decreased in both groups. Acute administration of metoprolol decreased infarction in control group and did not affect the CA-elicited cardiprotection. Accordingly, neither beta 1-AR-G(s)alpha-adenyly-1- cyclase signaling. stimulated with specific ligands, nor p-PKA/PICA ratios were affected after CA or CAR. Importantly. Western blot and immunofluorescence analyses revealed beta 2- and beta 3-AR protein enrichment in membranes in both experimental groups. We conclude that gradual cold acclimation results in a persisting increase of myocardial resistance to I/R injury without hypertension and hypertrophy. The cardioprotective phenotype is associated with unaltered adenylyl cyclase signaling and increased mitochondrial resistance to Ca2+-overload. The potential role of upregulated beta 2/beta 3-AR pathways remains to be elucidated. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address https://doi.org/10.1152/japplphysiol.00511.2019
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