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Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles

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    SYSNO ASEP0524235
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleConstant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles
    Author(s) Šebest, Peter (BFU-R) ORCID
    Ostatná, Veronika (BFU-R) RID, ORCID
    Fojta, Miroslav (BFU-R) RID, ORCID
    Daňhel, Aleš (BFU-R) RID, ORCID
    Number of authors4
    Article number113854
    Source TitleJournal of Electroanalytical Chemistry - ISSN 1572-6657
    Roč. 859, FEB 15 2020 (2020)
    Number of pages5 s.
    Publication formOnline - E
    Languageeng - English
    CountryCH - Switzerland
    Keywordsprotein ; voltammetry ; Bovine serum albumin
    Subject RIVCG - Electrochemistry
    OECD categoryElectrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
    R&D ProjectsGJ17-23634Y GA ČR - Czech Science Foundation (CSF)
    EF15_003/0000477 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingLimited access
    Institutional supportBFU-R - RVO:68081707
    UT WOS000521509300011
    EID SCOPUS85078030466
    DOI10.1016/j.jelechem.2020.113854
    AnnotationHighly sensitive peak H registered by constant-current chronopotentiometric stripping (CPS) analysis relates due to the catalytic hydrogen evolution reaction of proteins adsorbed on mercury-based surfaces including recently introduced silver amalgam particles (AgAPs). Since the peak H allows a structure-sensitive analysis of proteins, including those important in biomedicine and cancer research, it has been systematically studied by CPS analysis using AgAPs in this work. Conditions of AgAPs' electrodeposition on basal plane pyrolytic graphite electrode (bPGE) were optimized towards CPS analysis of model protein (bovine serum albumin, BSA) using peak H. Optimized condition for bPGE-AgAP preparation allowed sensitive CPS detection of either native or denatured BSA down to 10 nM concentration, a discrimination between both forms, and registration of the BSA thermal denaturation kinetic. Obtained results have confirmed the applicability of AgAPs in protein electroanalysis and may contribute to the further development of novel (bio)sensors or (bio)electrochemical applications using micro-volume batch, flow-through or lab-on-chip detection systems. (C) 2020 Elsevier B.V. All rights reserved.
    WorkplaceInstitute of Biophysics
    ContactJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Year of Publishing2021
    Electronic addresshttps://www.sciencedirect.com/science/article/abs/pii/S1572665720300370
Number of the records: 1