Number of the records: 1

Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles

SYSNO ASEP	0524235
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles
Author(s)	Šebest, Peter (BFU-R)_ORCID Ostatná, Veronika (BFU-R)_{RID, ORCID} Fojta, Miroslav (BFU-R)_{RID, ORCID} Daňhel, Aleš (BFU-R)_{RID, ORCID}
Number of authors	4
Article number	113854
Source Title	Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657 Roč. 859, FEB 15 2020 (2020)
Number of pages	5 s.
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	protein ; voltammetry ; Bovine serum albumin
Subject RIV	CG - Electrochemistry
OECD category	Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
R&D Projects	GJ17-23634Y GA ČR - Czech Science Foundation (CSF)
	EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	BFU-R - RVO:68081707
UT WOS	000521509300011
EID SCOPUS	85078030466
DOI	10.1016/j.jelechem.2020.113854
Annotation	Highly sensitive peak H registered by constant-current chronopotentiometric stripping (CPS) analysis relates due to the catalytic hydrogen evolution reaction of proteins adsorbed on mercury-based surfaces including recently introduced silver amalgam particles (AgAPs). Since the peak H allows a structure-sensitive analysis of proteins, including those important in biomedicine and cancer research, it has been systematically studied by CPS analysis using AgAPs in this work. Conditions of AgAPs' electrodeposition on basal plane pyrolytic graphite electrode (bPGE) were optimized towards CPS analysis of model protein (bovine serum albumin, BSA) using peak H. Optimized condition for bPGE-AgAP preparation allowed sensitive CPS detection of either native or denatured BSA down to 10 nM concentration, a discrimination between both forms, and registration of the BSA thermal denaturation kinetic. Obtained results have confirmed the applicability of AgAPs in protein electroanalysis and may contribute to the further development of novel (bio)sensors or (bio)electrochemical applications using micro-volume batch, flow-through or lab-on-chip detection systems. (C) 2020 Elsevier B.V. All rights reserved.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2021
Electronic address	https://www.sciencedirect.com/science/article/abs/pii/S1572665720300370

Number of the records: 1