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Study of permeabilization of bacterial membrane by electrochemical methods
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SYSNO ASEP 0524215 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Study of permeabilization of bacterial membrane by electrochemical methods Author(s) Čierna, M. (CZ)
Naumowicz, M. (PL)
Birošová, L. (SK)
Krahulec, J. (SK)
Sokolová, Romana (UFCH-W) RID, ORCID, SAI
Kolivoška, Viliam (UFCH-W) RID, ORCID
Sebechlebská, Táňa (UFCH-W) SAI, ORCID
Kielar, F. (TH)
Gál, M. (SK)Article number 113761 Source Title Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
Roč. 857, JAN 2020 (2020)Number of pages 5 s. Language eng - English Country CH - Switzerland Keywords antimicrobial peptides ; escherichia-coli ; carbon nutrition ; evolution ; ll-37 ; Voltammetry ; Cathelicidin ; Permeabilization ; E. coli Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GA18-09848S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UFCH-W - RVO:61388955 UT WOS 000518494300029 EID SCOPUS 85076881612 DOI 10.1016/j.jelechem.2019.113761 Annotation Selected electrochemical methods for the determination of possible membrane permeabilization in two Escherichia coli bacterial strains were examined. Escherichia coli, a gram-negative rod-shaped bacterium, is an important component of the mammalian intestinal microbiota. However, some strains of this bacterium are pathogenic and can cause a variety of diseases. Partial aim of this work was to identify beta-galactosidase enzyme as one of the possible factors characterizing disintegration of bacterial membrane. To permeabilize the bacterial membrane, cathelicidin LL-37, the body's own antimicrobial peptide, and membrane degradation by microwave radiation were used. In the investigation of structural changes in the bacterial membrane, square-wave voltammetry and chronopotentiometric dissolution analysis in combination with the mercury drop electrode and the glassy carbon electrode were used. UV/VIS spectrophotometry was used to indirectly track changes in bacterial cell structure. (C) 2019 The Authors. Published by Elsevier B.V. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2021 Electronic address 10.1016/j.jelechem.2019.113761
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