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Effects of ex situ chronopotentiometric analysis on stability of bovine serum albumin on mercury electrodes
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SYSNO ASEP 0523951 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effects of ex situ chronopotentiometric analysis on stability of bovine serum albumin on mercury electrodes Author(s) Ostatná, Veronika (BFU-R) RID, ORCID
West, R. M. (US)Number of authors 2 Article number 113884 Source Title Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
Roč. 860, 1.3.2020 (2020)Number of pages 7 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords hydrogen evolution ; proteins ; catalysis ; electrochemistry Subject RIV AH - Economics OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GA18-18154S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000519336100008 EID SCOPUS 85078696760 DOI 10.1016/j.jelechem.2020.113884 Annotation Constant current chronopotentiometric stripping (CPS) allows analysis of proteins based on measured peak H resulting from catalytic hydrogen evolution reaction. The technique is label-free and sensitive to the structure and stability of the protein adsorbed at the mercury electrode. Comparison of proteins must be carried out under the same conditions, including ionic strength, temperature, pH, and accumulation potential and time, as all of these factors can affect the protein stability and structure on the electrode surface. Here we show that for bovine serum albumin, uncontrolled disconnection of the cell after accumulation, as is necessary for ex situ CPS measurements, can cause an increased susceptibility to electric field-induced denaturation during the subsequent CPS measurement. This destabilization is attributed to oxidation of the Hg electrode during disconnection. For this reason, care much be taken when ex situ CPS measurement of protein is performed. (C) 2020 Elsevier B.V. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2021 Electronic address https://www.sciencedirect.com/science/article/abs/pii/S1572665720300679?via%3Dihub
Number of the records: 1