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Modification of Human Pericardium by Chemical Crosslinking
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SYSNO ASEP 0523850 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Modification of Human Pericardium by Chemical Crosslinking Author(s) Filová, Elena (FGU-C) RID, ORCID
Staňková, Ľubica (FGU-C) RID, ORCID
Eckhardt, Adam (FGU-C) RID, ORCID
Svobodová, Jana (FGU-C)
Musílková, Jana (FGU-C) RID, ORCID
Pala, J. (CZ)
Hadraba, Daniel (FGU-C) RID, ORCID, SAI
Brynda, Eduard (UMCH-V) RID
Koňařík, M. (CZ)
Pirk, J. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDSource Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 69, č. 1 (2020), s. 49-59Number of pages 11 s. Language eng - English Country CZ - Czech Republic Keywords human pericardium ; valve interstitial cells ; chemical crosslinking ; genipin ; glutaraldehyde Subject RIV FA - Cardiovascular Diseases incl. Cardiotharic Surgery OECD category Cardiac and Cardiovascular systems Subject RIV - cooperation Institute of Macromolecular Chemistry - Macromolecular Chemistry R&D Projects NV15-29153A GA MZd - Ministry of Health (MZ) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NV18-02-00422 GA MZd - Ministry of Health (MZ) Research Infrastructure Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support FGU-C - RVO:67985823 ; UMCH-V - RVO:61389013 UT WOS 000514830600004 EID SCOPUS 85081100461 DOI 10.33549/physiolres.934335 Annotation Autologous and allogenic human pericardia used as biomaterials for cardiovascular surgery are traditionally crosslinked with glutaraldehyde. In this work, we have evaluated the resistivity to collagenase digestion and the cytotoxicity of human pericardium crosslinked with various concentrations of glutaraldehyde in comparison with pericardium crosslinked by genipin, nordihydroguaiaretic acid, tannic acid, and in comparison with unmodified pericardium. Crosslinking retained the wavy-like morphology of native pericardium visualized by second harmonic generation microscopy. The collagenase digestion products were analyzed using SDS-PAGE, capillary electrophoresis, and a hydroxyproline assay. Glutaraldehyde and genipin crosslinking protected the native pericardium efficiently against digestion with collagenase III. Only low protection was provided by the other crosslinking agents. The cytotoxicity of crosslinked pericardium was evaluated using xCELLigence by monitoring the viability of porcine valve interstitial cells cultured in eluates from crosslinked pericardium. The highest cell index, reflecting both the number and the shape of the monitored cells was observed in eluates from genipin. Crosslinking pericardium grafts with genipin therefore seems to be a promising alternative procedure to the traditional crosslinking with glutaraldehyde, because it provides similarly high protection against degradation with collagenase, without cytotoxic effects. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address http://www.biomed.cas.cz/physiolres/pdf/2020/69_49.pdf
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