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A new pH sensor localized in the Golgi apparatus of Saccharomyces cerevisiae reveals unexpected roles of Vph1p and Stv1p isoforms
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SYSNO ASEP 0523841 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A new pH sensor localized in the Golgi apparatus of Saccharomyces cerevisiae reveals unexpected roles of Vph1p and Stv1p isoforms Author(s) Deschamps, A. (BE)
Colinet, A. S. (BE)
Zimmermannová, Olga (FGU-C) RID, ORCID
Sychrová, Hana (FGU-C) RID, ORCID
Morsomme, P. (BE)Article number 1881 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords Golgi ; pHluorin ; V-ATPase ; yeast ; STV1 ; VPH1 ; glucose availability Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA17-01953S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000515602400002 EID SCOPUS 85079069709 DOI 10.1038/s41598-020-58795-w Annotation The gradual acidification of the secretory pathway is conserved and extremely important for eukaryotic cells, but until now there was no pH sensor available to monitor the pH of the early Golgi apparatus in Saccharomyces cerevisiae. Therefore, we developed a pHluorin-based sensor for in vivo measurements in the lumen of the Golgi. By using this new tool we show that the cis- and medial-Golgi pH is equal to 6.6-6.7 in wild type cells during exponential phase. As expected, V-ATPase inactivation results in a near neutral Golgi pH. We also uncover that surprisingly Vph1p isoform of the V-ATPase is prevalent to Stv1p for Golgi acidification. Additionally, we observe that during changes of the cytosolic pH, the Golgi pH is kept relatively stable, mainly thanks to the V-ATPase. Eventually, this new probe will allow to better understand the mechanisms involved in the acidification and the pH control within the secretory pathway. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address https://doi.org/10.1038/s41598-020-58795-w
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