Number of the records: 1

The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A, a comparison with morphine-treated cells

SYSNO ASEP	0523837
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A, a comparison with morphine-treated cells
Author(s)	Ujčíková, Hana (FGU-C)_{RID, ORCID} Cechová, Kristína (FGU-C)_{RID, ORCID} Roubalová, Lenka (FGU-C)_{RID, ORCID, SAI} Brejchová, Jana (FGU-C)_{RID, ORCID} Kaufman, Jonáš (FGU-C) Holáň, Vladimír (UEM-P)_RID Svoboda, Petr (FGU-C)_{RID, ORCID}
Article number	577191
Source Title	Journal of Neuroimmunology. - : Elsevier - ISSN 0165-5728 Roč. 341, Apr 15 (2020)
Number of pages	14 s.
Language	eng - English
Country	NL - Netherlands
Keywords	morphine ; concanavalin A ; rat spleen lymphocytes ; proteomic analysis ; up-regulation of brain proteins
Subject RIV	ED - Physiology
OECD category	Physiology (including cytology)
Subject RIV - cooperation	Institute of Experimental Medicine - Genetics ; Molecular Biology
R&D Projects	GA19-03295S GA ČR - Czech Science Foundation (CSF)
	LTAUSA18110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	FGU-C - RVO:67985823 ; UEM-P - RVO:68378041
UT WOS	000521114100009
EID SCOPUS	85079849005
DOI	10.1016/j.jneuroim.2020.577191
Annotation	Morphine- and Concanavalin A-induced changes of protein composition of rat spleen lymphocytes were determined by high-resolution proteomic analysis, gel-free, label-free quantification, MaxLFQ. Stimulation by Con A resulted in a major reorganization of spleen cell protein composition evidenced by increased expression level of 94 proteins, 101 proteins were down-regulated (> 2-fold). Interestingly, among proteins that were up-regulated to the largest extent were the prototypical brain proteins as a neuron specific enolase, synapsin-1, brain acid-soluble protein-1 and myelin basic protein. Morphine-induced change was limited to no more than 5 up-regulated and 18 down-regulated proteins (> 2-fold).
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2021
Electronic address	https://doi.org/10.1016/j.jneuroim.2020.577191

Number of the records: 1