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Comparative analysis of chromosome 2A molecular organization in diploid and hexaploid wheat
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SYSNO ASEP 0523650 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparative analysis of chromosome 2A molecular organization in diploid and hexaploid wheat Author(s) Kaur, P. (AU)
Jindal, S. (IN)
Yadav, B. (IN)
Yadav, I. (IN)
Mahato, A. (IN)
Sharma, P. (IN)
Kaur, S. (IN)
Gupta, O. P. (IN)
Vrána, Jan (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Gill, B.S. (US)
Meyer, K. F. X. (DE)
Khurana, J. P. (IN)
Singh, N.K. (JP)
Chhuneja, P. (IN)
Singh, K. (US)Number of authors 17 Source Title Molecular Biology Reports. - : Springer - ISSN 0301-4851
Roč. 47, č. 3 (2020), s. 1991-2003Number of pages 13 s. Language eng - English Country NL - Netherlands Keywords Anchoring ; Chromosome 2A ; Diploid wheat ; Genetic map ; Linkage map ; Physical map Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000515965000001 EID SCOPUS 85079139829 DOI 10.1007/s11033-020-05295-9 Annotation Diploid A genome wheat species harbor immense genetic variability which has been targeted and proven useful in wheat improvement. Development and deployment of sequence-based markers has opened avenues for comparative analysis, gene transfer and marker assisted selection (MAS) using high throughput cost effective genotyping techniques. Chromosome 2A of wheat is known to harbor several economically important genes. The present study aimed at identification of genic sequences corresponding to full length cDNAs and mining of SSRs and ISBPs from 2A draft sequence assembly of hexaploid wheat cv. Chinese Spring for marker development. In total, 1029 primer pairs including 478 gene derived, 501 SSRs and 50 ISBPs were amplified in diploid A genome species Triticum monococcum and T. boeoticum identifying 221 polymorphic loci. Out of these, 119 markers were mapped onto a pre-existing chromosome 2A genetic map consisting of 42 mapped markers. The enriched genetic map constituted 161 mapped markers with final map length of 549.6 cM. Further, 2A genetic map of T. monococcum was anchored to the physical map of 2A of cv. Chinese Spring which revealed several rearrangements between the two species. The present study generated a highly saturated genetic map of 2A and physical anchoring of genetically mapped markers revealed a complex genetic architecture of chromosome 2A that needs to be investigated further. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address http://doi.org/10.1007/s11033-020-05295-9
Number of the records: 1