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Fine Mapping of Lr49 Using 90K SNP Chip Array and Flow-Sorted Chromosome Sequencing in Wheat
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SYSNO ASEP 0523435 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fine Mapping of Lr49 Using 90K SNP Chip Array and Flow-Sorted Chromosome Sequencing in Wheat Author(s) Nsabiyera, V. (AU)
Baranwal, D. (AU)
Qureshi, N. (US)
Kay, P. (AU)
Forrest, K. L. (AU)
Valárik, Miroslav (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Hayden, M. J. (AU)
Bariana, H. (AU)
Bansal, U. (AU)Number of authors 10 Article number 1787 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 10, FEB 4 (2020)Number of pages 11 s. Language eng - English Country CH - Switzerland Keywords adult plant resistance ; chromosome sorting ; Infinium iSelect 90K SNP array ; leaf rust ; marker assisted breeding Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000515758000001 EID SCOPUS 85079645049 DOI 10.3389/fpls.2019.01787 Annotation Leaf rust, caused by Puccinia triticina, threatens global wheat production due to the constant evolution of virulent pathotypes that defeat commercially deployed all stage-resistance (ASR) genes in modern cultivars. Hence, the deployment of combinations of adult plant resistance (APR) and ASR genes in new wheat cultivars is desirable. Adult plant resistance gene Lr49 was previously mapped on the long arm of chromosome 4B of cultivar VL404 and flanked by microsatellite markers barc163 (8.1 cM) and wmc349 (10.1 cM), neither of which was sufficiently closely linked for efficient marker assisted selection. This study used high-density SNP genotyping and flow sorted chromosome sequencing to fine-map the Lr49 locus as a starting point to develop a diagnostic marker for use in breeding and to clone this gene. Marker sunKASP_21 was mapped 0.4 cM proximal to Lr49, whereas a group of markers including sunKASP_24 were placed 0.6 cM distal to this gene. Testing of the linked markers on 75 Australian and 90 European cultivars with diverse genetic backgrounds showed that sunKASP_21 was most strongly associated with Lr49. Our results also show that the Lr49 genomic region contains structural variation relative to the reference stock Chinese Spring, possibly an inverted genomic duplication, which introduces a new set of challenges for the Lr49 cloning. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address http://doi.org/10.3389/fpls.2019.01787
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