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Effects of post-mortem and physical degradation on RNA integrity and quality
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SYSNO ASEP 0523050 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve SCOPUS Title Effects of post-mortem and physical degradation on RNA integrity and quality Author(s) Šídová, Monika (BTO-N) RID
Tománková, Silvie (BTO-N) RID
Abaffy, Pavel (BTO-N)
Kubista, Mikael (BTO-N) RID
Šindelka, Radek (BTO-N) RIDNumber of authors 5 Source Title Biomolecular Detection and Quantification - ISSN 2214-7535
Roč. 5, September 2015 (2015), s. 3-9Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords Degradation ; RNA integrity ; RNA quality ; rqi Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BTO-N - RVO:86652036 EID SCOPUS 84943627995 DOI 10.1016/j.bdq.2015.08.002 Annotation The precision and reliability of quantitative nucleic acid analysis depends on the quality of the sample analyzed and the integrity of the nucleic acids. The integrity of RNA is currently primarily assessed by the analysis of ribosomal RNA, which is the by far dominant species. The extrapolation of these results to mRNAs and microRNAs, which are structurally quite different, is questionable. Here we show that ribosomal and some nucleolar and mitochondrial RNAs, are highly resistant to naturally occurring post-mortem degradation, while mRNAs, although showing substantial internal variability, are generally much more prone to nucleolytic degradation. In contrast, all types of RNA show the same sensitivity to heat. Using qPCR assays targeting different regions of mRNA molecules, we find no support for 5' or 3' preferentiality upon post-mortem degradation. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S2214753515300048?via%3Dihub
Number of the records: 1