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THE EFFECT OF SIMULATED BODY FLUID ON THE GROWTH AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS CULTURED ON NANOSTRUCTURED Ti6Al4V SURFACES
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SYSNO ASEP 0522951 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title THE EFFECT OF SIMULATED BODY FLUID ON THE GROWTH AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS CULTURED ON NANOSTRUCTURED Ti6Al4V SURFACES Author(s) Filová, Eva (UEM-P) RID, ORCID
Voltrová, Barbora (UEM-P)
Jarolímová, P. (CZ)
Matějka, Roman (FGU-C) RID, ORCID, SAI
Lukášová, Věra (UEM-P)
Fojt, J. (CZ)
Joska, L. (CZ)
Daniel, M. (CZ)Source Title NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application. - Ostrava : Tanger Ltd., 2019 - ISBN 978-808729489-5 Pages s. 300-305 Number of pages 6 s. Publication form Print - P Action NANOCON 2018 -International Conference on Nanomaterials - Research and Application /10./ Event date 17.10.2018 - 19.10.2018 VEvent location Brno Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords anodic oxidation ; hydroxyapatite ; mesenchymal stem cell Subject RIV EI - Biotechnology ; Bionics OECD category Biomaterials (as related to medical implants, devices, sensors) Subject RIV - cooperation Institute of Physiology - Biotechnology ; Bionics R&D Projects LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA16-14758S GA ČR - Czech Science Foundation (CSF) Institutional support UEM-P - RVO:68378041 ; FGU-C - RVO:67985823 UT WOS 000513131900052 EID SCOPUS 85062949908 Annotation TI6Al4V is a standard medical titanium alloy. In this study, we compared ground Ti6Al4V discs with nanostructured Ti6Al4V discs prepared using the anodic oxidation method at 30V. Subsequently, both the nanostructured and ground discs were exposed to simulated body fluid (SBF) for 7 days in order to form a hydroxyapatite-like layer. Human mesenchymal stem cells (hMSC) were seeded on the discs in osteogenic differentiation medium and cultivated for 21 days. hMSCs were tested for metabolic activity (MTS assay) and rate of proliferation. The synthesis of type I collagen was evaluated using immunohistochemical staining and confocal microscopy analysis. All samples supported cell adhesion and proliferation. The highest amount of type I collagen was found on nanostructured (N) and ground Ti6Al4V exposed to SBF (TiH) surfaces. Thus, SBF and anodic oxidation positively influenced the growth and osteogenic differentiation of hMSCs. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2020
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