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The effect of mitochondrially targeted anticancer agents on mitochondrial (super)complexes
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SYSNO ASEP 0522123 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve SCOPUS Title The effect of mitochondrially targeted anticancer agents on mitochondrial (super)complexes Author(s) Vondrusová, Magdaléna (BTO-N) RID
Bezawork-Geleta, A. (AU)
Sachaphibulkij, K. (AU)
Truksa, Jaroslav (BTO-N) RID, ORCID
Neužil, Jiří (BTO-N) RIDNumber of authors 5 Source Title Methods in molecular biology - ISSN 1940-6029
Roč. 1265, č. 2015 (2015), s. 195-208Number of pages 14 s. Language eng - English Country US - United States Keywords antineoplastic agent ; mitochondrial protein ; multienzyme complex Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology Method of publishing Limited access Institutional support BTO-N - RVO:86652036 EID SCOPUS 84958608088 DOI 10.1007/978-1-4939-2288-8_15 Annotation The mitochondrial respiratory chain is organized into dynamic high molecular weight complexes that associate to form supercomplexes. The function of these SCs is to minimize the production of reactive oxygen species (ROS) generated during electron transfer within them and to efficiently transfer electrons to complex IV. These supra-molecular structures as well as whole mitochondria are stress-responsive and respond to mitochondrially targeted anti-cancer agent by destabilization and induction of massive production of ROS leading to apoptosis. We have recently developed mitochondrially targeted anti-cancer agents epitomized by the mitochondrially targeted analogue of the redox-silent compound vitamin E succinate, which belongs to the group of agents that kill cancer cells via their mitochondria-destabilizing activity, referred to as mitocans. To understand the molecular mechanism of the effect of such agents, the use of native blue gel electrophoresis and clear native electrophoresis coupled with in-gel activity assays, are methods of choice. The relevant methodology is described in this chapter. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2020 Electronic address https://link.springer.com/protocol/10.1007%2F978-1-4939-2288-8_15
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