Number of the records: 1

PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION

SYSNO ASEP	0520853
Document Type	M - Monograph Chapter
R&D Document Type	Monograph Chapter
Title	Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature
Author(s)	Nebesářová, Jana (BC-A)_{RID, ORCID}
Number of authors	1
Source Title	PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION. - TOTOWA : HUMANA PRESS INC., 2019 / Cvrčková F. ; Žárský Viktor - ISSN 1064-3745 - ISBN 978-1-4939-9469-4
Pages	s. 63-76
Series	Methods in Molecular Biology
Number of pages	13 s.
Number of pages	380
Publication form	Print - P
Language	eng - English
Country	US - United States
Keywords	surfaces ; sem ; fixation ; High-resolution scanning electron microscope ; Plant sample ; Chemical fixation ; Dehydration ; Critical point drying ; t-Butanol freeze drying ; Metal coating ; Sample storage
Subject RIV	EA - Cell Biology
OECD category	Cell biology
R&D Projects	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BC-A - RVO:60077344
UT WOS	000487932800005
EID SCOPUS	85066777742
DOI	10.1007/978-1-4939-9469-4_4
Annotation	In this chapter, conventional techniques are described for the preparation of plant samples at room temperature before examination in the high resolution scanning electron microscopy. Protocols are given on how to collect, to fix, to dehydrate, and to dry plant samples. Subsequently, it is described how to stick them to stubs and cover with a thin conductive layer. These methods are suitable for a wide variety of plant specimens, ranging from microalgae to higher plants.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2020
Electronic address	https://link.springer.com/protocol/10.1007/978-1-4939-9469-4_4

Number of the records: 1