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The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography
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SYSNO ASEP 0518846 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography Author(s) Vojtová, L. (CZ)
Zikmund, T. (CZ)
Pavliňáková, V. (CZ)
Šalplachta, J. (CZ)
Kalašová, D. (CZ)
Prosecká, Eva (UEM-P)
Brtníková, J. (CZ)
Žídek, J. (CZ)
Pavliňák, D. (CZ)
Kaiser, J. (CZ)Source Title Journal of Microscopy. - : Wiley - ISSN 0022-2720
Roč. 273, č. 3 (2019), s. 169-177Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords biopolymeric scaffold ; mesenchymal stem cells ; SEM/EDX Subject RIV EI - Biotechnology ; Bionics OECD category Biomaterials (as related to medical implants, devices, sensors) Method of publishing Limited access Institutional support UEM-P - RVO:68378041 UT WOS 000458426100002 EID SCOPUS 85057052831 DOI 10.1111/jmi.12771 Annotation This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent - heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2020 Electronic address https://onlinelibrary.wiley.com/doi/abs/10.1111/jmi.12771
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