Number of the records: 1
Antibacterial and antifungal activity of defensins from the Australian paralysis tick, Ixodes holocyclus
- 1.
SYSNO ASEP 0518834 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Antibacterial and antifungal activity of defensins from the Australian paralysis tick, Ixodes holocyclus Author(s) Cabezas-Cruz, A. (FR)
Tonk, M. (DE)
Bleackley, M.R. (AU)
Valdés, James J. (BC-A) RID, ORCID
Barrero, R. A. (AU)
Hernández-Jarguín, A. (ES)
Moutailler, S. (FR)
Vilcinskas, A. (DE)
Richard-Forget, F. (FR)
Anderson, M.A. (DE)
Rodriguez-Valle, M. (AU)Number of authors 11 Article number 101269 Source Title Ticks and Tick-borne Diseases - ISSN 1877-959X
Roč. 10, č. 6 (2019)Number of pages 9 s. Publication form Online - E Language eng - English Country DE - Germany Keywords antimicrobial peptides ; protein ; sequence ; gene ; identification ; prediction ; energetics ; mechanism ; evolution ; immunity ; Ticks ; Defensins ; Antibacterial activity ; Antifungal activity ; Ixodes holocyclus Subject RIV FN - Epidemiology, Contagious Diseases ; Clinical Immunology OECD category Infectious Diseases Method of publishing Limited access Institutional support BC-A - RVO:60077344 UT WOS 000484783700018 EID SCOPUS 85070885430 DOI 10.1016/j.ttbdis.2019.101269 Annotation Tick innate immunity involves humoral and cellular responses. Among the humoral effector molecules in ticks are the defensins which are a family of small peptides with a conserved gamma-core motif that is crucial for their antimicrobial activity. Defensin families have been identified in several hard and soft tick species. However, little is known about the presence and antimicrobial activity of defensins from the Australian paralysis tick Ixodes holocyclus. In this study the I. holocyclus transcriptome was searched for the presence of defensins. Unique and non-redundant defensin sequences were identified and designated as holosins 1 5. The antimicrobial activity of holosins 2 and 3 and of the predicted y-cores of holosins 1-4 (HoloTickCores 1-4), was assessed using Gram-negative and Gram-positive bacteria as well as the fungus Fusarium graminearum and the yeast Candida albicans. All holosins had molecular features that are conserved in other tick defensins. Furthermore holosins 2 and 3 were very active against the Gram-positive bacteria Staphylococcus aureus and Listeria grayi. Holosins 2 and 3 were also active against F. graminearum and C. albicans and 5 mu M of peptide abrogate the growth of these microorganisms. The activity of the synthetic gamma-cores was lower than that of the mature defensins apart from HoloTickCore 2 which had activity comparable to mature holosin 2 against the Gram-negative bacterium Escherichia coli. This study reveals the presence of a multigene defensin family in I. holocyclus with wide antimicrobial activity. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/abs/pii/S1877959X19302183?via%3Dihub
Number of the records: 1