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Monitoring of Hydroxyl Radical Induced Oxidation of Yeast Cells Using Biological Autoluminescence
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SYSNO ASEP 0518815 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Monitoring of Hydroxyl Radical Induced Oxidation of Yeast Cells Using Biological Autoluminescence Author(s) Bereta, M. (SK)
Teplan, M. (SK)
Vahalová, Petra (URE-Y)
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 4 Source Title Proceedings of the 12th International Conference on Measurement, MEASUREMENT 2019. - Bratislava : IEEE, 2019 / Maňka J. ; Švehlíková J. ; Witkovský V. ; Frollo I. - ISBN 978-809726293-8 Pages s. 248-251 Number of pages 4 s. Publication form Print - P Action 12th International Conference on Measurement Event date 27.05.2019 - 29.05.2019 VEvent location Smolenice Country SK - Slovakia Event type WRD Language eng - English Country SK - Slovakia Keywords Hydroxyl Radical ; Biological Autoluminescence ; Reactive Oxygen Species Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Electrical and electronic engineering R&D Projects GA17-11898S GA ČR - Czech Science Foundation (CSF) GA18-23597S GA ČR - Czech Science Foundation (CSF) Institutional support URE-Y - RVO:67985882 UT WOS 000502830700055 EID SCOPUS 85070971262 DOI 10.23919/measurement47340.2019.8779909 Annotation It is known that the excessive oxidative processes (oxidative stress) in organisms are correlated with various diseases. Here we show the non-invasive monitoring of oxidative stress of yeast Saccharomyces cerevisiae cells using biological autoluminescence (BAL). The physical nature of this specific phenomenon is briefly introduced, and the results of BAL measurements from yeast cell solutions with the presence of hydrogen peroxide and ferrous ions are presented. The results confirm the assumption that the BAL intensity is mediated by hydroxyl radical, produced by the Fenton reaction, which causes oxidation of cells and initiates biochemical reactions leading to BAL generation. Since oxidative stress induced BAL from yeast cells has not been widely analyzed before in the literature, our results could be a useful contribution to this field of research Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2020
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