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Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting

SYSNO ASEP	0518558
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting
Author(s)	Búzová, Diana (UEK-B)_{SAI, ORCID, RID} Literáková, Petra (UEK-B)_{ORCID, RID, SAI} Fedorko, Jan (UEK-B)_{SAI, ORCID} Kaštánek, P. (CZ) Červený, Jan (UEK-B)_{RID, ORCID, SAI}
Source Title	Book of abstract: 9th European Symposium on Plant Lipids. - Marseille, 2019 / Beisson Fred ; Li-Beisson Yonghua S. 52-53
Number of pages	1 s.
Action	9th European Symposium on Plant Lipids
Event date	07.07.2019 - 10.07.2019
VEvent location	Marseille
Country	FR - France
Event type	EUR
Language	eng - English
Country	FR - France
Keywords	breeding methods ; lipids accumulation ; fluorescence-activated cell sorting ; microalgae
R&D Projects	TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
	LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2015055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEK-B - RVO:86652079
Annotation	Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting. The marine microorganism belonging to the family Thraustochytriidae are group of non-photosynthetic marine protists. These unicellular, heterotrophic eukaryotes are commonly found in seawater and sediments, with the highest abundance in nutrient-rich areas, such as mangrove forests. Thraustochytrids are reported to be efficient producers of polyunsaturated fatty acids (PUFA) including the n-3 series, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). These organisms are considered as one of the potential alternative sources of PUFA for commercial and industrial exploitation. We developed advanced cultivation strategy and breeding method, and applied them on one particular Thraustochytrid strain Schizochytrium AN4 for optimization of lipids accumulation. The breeding method aims for selection of the hyper producing subpopulation using high-throughput fluorescence-activated cell sorting (FACS) in combination with imaging flow cytometry (IFC) and multiparametric discrimination the cells with both high lipid content and metabolic activity. The method was tested for negative influence on post-sorting cell viability possibly caused by the sorting technique procedure (e.g. high pressure, laser beam exposure, electro-magnetic field, toxic effects of both fluorescent marker and fluidic system medium composition) with no effect compared to non-sorted control sample.
Workplace	Global Change Research Institute
Contact	Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268
Year of Publishing	2020

Number of the records: 1