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Chromosome Painting Facilitates Anchoring Reference Genome Sequence to Chromosomes In Situ and Integrated Karyotyping in Banana (Musa Spp.)
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SYSNO ASEP 0518420 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chromosome Painting Facilitates Anchoring Reference Genome Sequence to Chromosomes In Situ and Integrated Karyotyping in Banana (Musa Spp.) Author(s) Šimoníková, Denisa (UEB-Q) ORCID
Němečková, Alžběta (UEB-Q) ORCID
Karafiátová, Miroslava (UEB-Q) RID, ORCID
Uwimana, B. (UG)
Swennen, R. (BE)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Hřibová, Eva (UEB-Q) RID, ORCIDNumber of authors 7 Article number 1503 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 10, NOV 20 (2019)Number of pages 12 s. Language eng - English Country CH - Switzerland Keywords repetitive dna-sequences ; flow-cytometric analysis ; ribosomal-rna genes ; 5s rdna ; fish ; hybridization ; wheat ; identification ; localization ; acuminata ; banana ; chromosome identification ; fluorescence in situ hybridization ; molecular karyotype ; Musa ; oligo painting FISH Subject RIV EB - Genetics ; Molecular Biology OECD category Agricultural biotechnology and food biotechnology R&D Projects GA19-20303S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000500980000001 EID SCOPUS 85076689140 DOI 10.3389/fpls.2019.01503 Annotation Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis DH Pahang to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address http://dx.doi.org/10.3389/fpls.2019.01503
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