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Label-free electrochemical analysis of purine nucleotides and nucleobases at disposable carbon electrodes in microliter volumes

SYSNO ASEP	0518362
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Label-free electrochemical analysis of purine nucleotides and nucleobases at disposable carbon electrodes in microliter volumes
Author(s)	Hasoň, Stanislav (BFU-R)_{RID, ORCID} Fojta, Miroslav (BFU-R)_{RID, ORCID} Ostatná, Veronika (BFU-R)_{RID, ORCID}
Number of authors	3
Article number	113252
Source Title	Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657 Roč. 847, AUG 15 2019 (2019)
Number of pages	7 s.
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	dna bases ; pencil electrodes ; graphene ; graphite
Subject RIV	CG - Electrochemistry
OECD category	Analytical chemistry
R&D Projects	GA17-08971S GA ČR - Czech Science Foundation (CSF)
	EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	BFU-R - RVO:68081707
UT WOS	000482532200065
DOI	10.1016/j.jelechem.2019.113252
Annotation	We analyzed purine nucleotides in oligodeoxynucleotides (ODN) and nucleobase residues acid-hydrolyzed ODN at nanomolar concentrations in few tens of microliters of solution at commercially available unmodified graphite pencil electrodes, screen printed graphite electrodes (SPGEs) and SPGEs modified by graphene, single- and multi-walled carbon nanotubes. Our results suggest that specific properties of different graphite-based electrodes have significant impact on ODN analysis. Nanostructure-modified electrodes produced higher signals due to purine nucleobase oxidation but also higher background currents. The unmodified disposable carbon electrodes allowed analysis of both free nucleobases and ODNs. Our results show that unmodified SPGEs represent a good compromise for signal/noise ratio. Electrooxidation of purines at these electrodes was only little affected by the presence of pyrimidines in contrast to the pencil graphite electrode.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2020
Electronic address	https://www.sciencedirect.com/science/article/pii/S157266571930520X?via%3Dihub

Number of the records: 1