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Influence of the lengths of thymine, cytosine, and adenine stretches on the two-dimensional condensation of oligodeoxynucleotides at mercury silver amalgam electrode surfaces

SYSNO ASEP	0518314
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Influence of the lengths of thymine, cytosine, and adenine stretches on the two-dimensional condensation of oligodeoxynucleotides at mercury silver amalgam electrode surfaces
Author(s)	Hasoň, Stanislav (BFU-R)_{RID, ORCID} Pivoňková, Hana (BFU-R)_ORCID Fojta, Miroslav (BFU-R)_{RID, ORCID}
Number of authors	3
Article number	113364
Source Title	Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657 Roč. 849, SEP 15 2019 (2019)
Number of pages	13 s.
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	single-stranded-dna ; interfacial behavior ; phase transients ; nucleic-acid ; electrochemical-behavior ; dipole-moments
Subject RIV	CG - Electrochemistry
OECD category	Analytical chemistry
R&D Projects	GA16-01625S GA ČR - Czech Science Foundation (CSF)
	EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	BFU-R - RVO:68081707
UT WOS	000495482900018
DOI	10.1016/j.jelechem.2019.113364
Annotation	Two-dimensional (2D) condensation of nucleic acid components at mercury electrodes around the potential of zero charge has been known for decades. In our previous pilot study, we reported on 2D condensation of homopyrimidine oligodeoxynucleotides (ODNs) at the hanging mercury drop electrode (HMDE) in negative potential region. In this work we focused in detail on the effect of the homopyrimidine ODN length (from 3- to 50-mers) on the formation and properties of 2D condensed films at the HMDE. To elicit possible orientation of the homopyrimidine ODN molecules in their 2D condensed layers (i.e. surface concentration of the ODN molecules and the cross-section areas per adsorbed ODN molecules) we used a model in which the maximum surface concentration of the adsorbed ODN molecules is determined from linear dependences of the square of capacitance pit width on temperature. Effect of the length of homoadenine ODNs on their adsorption/desorption processes were studied for comparison, as well as effects of ionic strength and base protonation. Further we focused on the effects of the length of periodically repeated homothymine/homocytosine stretches in 30-mer ODNs on their interfacial behavior at the negatively charged HMDE. Experiments with binary mixtures of noncomplementary and complementary 15-mer homo-ODNs revealed dominance of the homopyrimidine, particularly cytosine, ODN responses in such competitive arrangement. The usefulness of solid silver amalgam electrode for this type of studies is demonstrated. Finally, potential applications of our results in label-free electrochemical analysis of alternative DNA structures are briefly discussed. (C) 2019 Elsevier B.V. All rights reserved.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2020
Electronic address	https://www.sciencedirect.com/science/article/pii/S1572665719306320?via%3Dihub

Number of the records: 1