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Rapid screening of very long-chain fatty acids from microorganisms
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SYSNO ASEP 0518107 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Rapid screening of very long-chain fatty acids from microorganisms Author(s) Řezanka, Tomáš (MBU-M) ORCID
Vítová, Milada (MBU-M) RID, ORCID
Lukavský, Jaromír (BU-J) RID, ORCID
Nedbalová, L. (CZ)
Kolouchová, I. (CZ)Article number UNSP 460365 Source Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1605, NOV 8 2019 (2019)Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords High resolution tandem mass spectrometr ; Very long-chain fatty acids ; GC-MS Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Institute of Botany R&D Projects GA17-00027S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 ; BU-J - RVO:67985939 UT WOS 000500389200017 EID SCOPUS 85070227846 DOI 10.1016/j.chroma.2019.460365 Annotation The analysis of triacylglycerols and phospholipids - phosphatidylcholines allowed the use of shotgun lipidomics to identify very long-chain fatty acids and very long-chain polyunsaturated fatty acids in microalgae. These fatty acids were determined in triacylglycerols by positive electrospray ionization of neutral loss scans of different fatty acids, e.g. 24:0, 24:19, 1 omega 9, 24:6 omega 3, 26:0, 26:1 omega 9, 28:0, 28:1 omega 9, 28:2 omega 6 and 28:8 omega 3. Likewise, very long-chain fatty acids in phosphatidylcholines were identified by negative electrospray ionization mass spectrometry in the selected ion-monitoring of the two most important ions (R1COO- and R2COO-). The limit of detection was determined at 10 nmol/L (similar to 11 pg/mu L) in triacylglycerols and 8.6 nmoles/L (similar to 8 pg/mu L) in phosphatidylcholines. The use of liquid chromatography-mass spectrometry is suitable for very long-chain polyunsaturated fatty acids with up to 8 double bonds due to the time of analysis as well as for reasons of lower thermal stability of polyunsaturated fatty acids towards saturated fatty acids, but gas chromatography-mass spectrometry is better suited for the analysis of saturated very long-chain fatty acids Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S0021967319307496?via%3Dihub
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