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Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells
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SYSNO ASEP 0517567 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells Author(s) Doubková, Martina (FGU-C) ORCID, RID
Němčáková, Ivana (FGU-C) RID, ORCID
Jirka, Ivan (UFCH-W) RID, ORCID
Březina, V. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDArticle number 3583 Source Title Materials. - : MDPI
Roč. 12, č. 21 (2019)Number of pages 13 s. Language eng - English Country CH - Switzerland Keywords silicalite-1 ; zeolite ; coating ; MG-63 cells ; osteoblasts ; biocompatibility Subject RIV EI - Biotechnology ; Bionics OECD category Biomaterials (as related to medical implants, devices, sensors) Subject RIV - cooperation J. Heyrovsky Institute of Physical Chemistry - Physical ; Theoretical Chemistry R&D Projects GA16-02681S GA ČR - Czech Science Foundation (CSF) GA19-02891S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support FGU-C - RVO:67985823 ; UFCH-W - RVO:61388955 UT WOS 000502798800124 EID SCOPUS 85074658438 DOI 10.3390/ma12213583 Annotation Silicalite-1 is a purely siliceous form of zeolite, which does not contain potentially harmful aluminum in its structure as opposed to ZSM-5 aluminosilicate types of zeolite. This paper reports on a study of a silicalite-1 film, deposited on a silicon Si(100) substrate, as a potential anti-corrosive and biocompatible coating for orthopaedic implants. Silicalite-1 film was prepared in situ on the surface of Si(100) wafers using a reaction mixture of tetrapropyl-ammonium hydroxide (TPAOH), tetraethyl-orthosilicate (TEOS), and diH2O. The physico-chemical properties of the obtained surface were characterized by means of X-ray photoelectron spectroscopy, water contact angle measurement, atomic force microscopy, and scanning electron microscopy. The biocompatibility was assessed by interaction with the MG-63 cell line (human osteosarcoma) in terms of cell adhesion, morphology, proliferation, and viability. The synthesized silicalite-1 film consisted of two layers (b- and a, b-oriented crystals) creating a combination of micro- and nano-scale surface morphology suitable for cell growth. Despite its hydrophobicity, the silicalite-1 film increased the number of initially adhered human osteoblast-like MG-63 cells and the proliferation rate of these cells. The silicalite-1 film also improved the cell viability in comparison with the reference Si(100) substrate. It is therefore a promising candidate for coating of orthopaedic implants. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2020 Electronic address https://doi.org/10.3390/ma12213583
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