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Spinning disk-based superresolution microscopy for subdiffraction structured illumination imaging of living cells
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SYSNO ASEP 0517466 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Spinning disk-based superresolution microscopy for subdiffraction structured illumination imaging of living cells Author(s) Lunov, Oleg (FZU-D) ORCID
Smolková, Barbora (FZU-D) ORCID
Lynnyková, Anna (FZU-D) RID
Uzhytchak, Mariia (FZU-D) ORCID
Kubinová, Šárka (FZU-D) RID, ORCID
Dejneka, Alexandr (FZU-D) RID, ORCIDNumber of authors 6 Source Title Jemná mechanika a optika. - : Fyzikální ústav AV ČR, v. v. i. - ISSN 0447-6441
Roč. 64, č. 5 (2019), s. 127-129Number of pages 3 s. Publication form Print - P Language eng - English Country CZ - Czech Republic Keywords superresolution microscopy ; structured illumination microscopy (SIM) ; lysosomes ; mitochondria Subject RIV BO - Biophysics OECD category Biophysics R&D Projects LO1409 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support FZU-D - RVO:68378271 Annotation Superresolution microscopy enables to see previously hidden details of cellular structures. However, it requires to use high irradiation intensities that may cause artefacts and photodamage of fragile biological samples. Newly developed confocal technique and Olympus super resolution (OSR), that combines spinning disk confocal with structured illumination microscopy, represents a reliable and fast superresolution avoiding photodamage. We demonstrate an OSR microscope platform, that enables subsecond, multicolour data acquisition. It also provides access to subdiffraction structured illumination imaging. We show that OSR allows live-cell experiments without any noticeable cellular damage. OSR system has an improved lateral (∼2) and axial (∼3) resolution compared with conventional confocal imaging. Moreover, OSR is compatible with both fixed and live cell imaging. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2020 Electronic address https://jmo.fzu.cz/sites/jmo.fzu.cz/files/oldweb/2019/2019-05/jmo_19_05_obsah.pdf
Number of the records: 1