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3D analysis of capillary network in skeletal muscle of obese insulin-resistant mice

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    SYSNO ASEP0517438
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    Title3D analysis of capillary network in skeletal muscle of obese insulin-resistant mice
    Author(s) Umek, N. (SI)
    Horvat, S. (SI)
    Cvetko, E. (SI)
    Kreft, M. (SI)
    Janáček, Jiří (FGU-C) RID, ORCID
    Kubínová, Lucie (FGU-C) RID, ORCID
    Pintaric, T.S. (SI)
    Eržen, I. (SI)
    Source TitleHistochemistry and Cell Biology - ISSN 0948-6143
    Roč. 152, č. 5 (2019), s. 323-331
    Number of pages9 s.
    Languageeng - English
    CountryDE - Germany
    Keywordsobesity ; insulin resistence ; capillary network ; 3D analysis ; skeletal muscle ; fibre type
    Subject RIVFP - Other Medical Disciplines
    OECD categoryAnatomy and morphology (plant science to be 1.6)
    R&D ProjectsLM2015062 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    EF16_013/0001775 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Research InfrastructureCzech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i.
    Method of publishingLimited access
    Institutional supportFGU-C - RVO:67985823
    UT WOS000495293400002
    EID SCOPUS85072132564
    DOI10.1007/s00418-019-01810-7
    AnnotationIn obesity, the skeletal muscle capillary network regresses and the insulin-mediated capillary recruitment is impaired. However, it has been shown that in the early stage of advanced obesity, an increased functional vascular response can partially compensate for other mechanisms of insulin resistance. The present study aimed to investigate the changes in the capillary network around individual muscle fibres during the early stage of obesity and insulin resistance in mice using 3D analysis. Capillaries and muscle fibres of the gluteus maximus muscles of seven high-fat-diet-induced obese and insulin-resistant mice and seven age-matched lean healthy mice were immunofluorescently labelled in thick transverse muscle sections. Stacks of images were acquired using confocal microscope. Capillary network characteristics were estimated by methods of quantitative image analysis. Muscle fibre typing was performed by histochemical analysis of myosin heavy chain isoforms on thin serial sections of skeletal muscle. Capillary length per muscle fibre length and capillary length per muscle fibre surface were increased by 27% and 23%, respectively, around small muscle fibres in obese mice, while there were no significant comparative differences around large fibres of obese and lean mice. Furthermore, the capillarization was larger around small compared to large fibres and there was a shift toward fast type myosin heavy chain isoforms, with no significant changes in muscle fibre diameters, tortuosity and anisotropy in obese mice. Overall, the results show that obese insulin-resistant mice have selective increase in capillarization around small predominantly intermediate muscle fibres, which is most likely related to the impaired glucose metabolism characteristic of type 2 diabetes.
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Year of Publishing2020
    Electronic addresshttps://link.springer.com/article/10.1007%2Fs00418-019-01810-7
Number of the records: 1