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BSA-Polysaccharide Interactions at Negatively Charged Electrode Surface. Effects of Current Density.
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SYSNO ASEP 0511698 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title BSA-Polysaccharide Interactions at Negatively Charged Electrode Surface. Effects of Current Density. Author(s) Černocká, Hana (BFU-R) RID, ORCID
Izadi, Nasim (BFU-R) ORCID
Ostatná, Veronika (BFU-R) RID, ORCID
Strmečki, S. (HR)Number of authors 4 Source Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 31, č. 10 (2019), s. 2007-2011Number of pages 5 s. Publication form Print - P Language eng - English Country DE - Germany Keywords bovine serum-albumin ; nonconjugated proteins ; alginate ; constant ; peptides Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000491476600021 DOI 10.1002/elan.201900231 Annotation Constant current chronopotentiometric stripping (CPS) peak H due to catalytic hydrogen evolution reaction on Hg-containing electrodes appeared useful in the analysis of protein complexes with single-stranded and double-stranded DNA as well as with peptides. In dependence on stripping current (I-str), structural transition of the protein alone or in complexes can be followed as a result of the protein exposure to electric field effects. For the first time we show here that the CPS analysis can be used for the study of the interaction of BSA with a polysaccharide namely sodium alginate (SA). BSA-SA complex formation was accompanied by the shift of the structural transition of BSA to lowerI-str intensities. Another polysaccharide dextran did not alter I-str-dependent structural transition of BSA. BSA-SA complex can be disturbed by an electric field effect or high ionic strength confirming the electrostatic nature of BSA-SA interaction. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020 Electronic address https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201900231
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