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The Legacy of Sexual Ancestors in Phenotypic Variability, Gene Expression, and Homoeolog Regulation of Asexual Hybrids and Polyploids
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SYSNO ASEP 0510908 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Legacy of Sexual Ancestors in Phenotypic Variability, Gene Expression, and Homoeolog Regulation of Asexual Hybrids and Polyploids Author(s) Bartoš, Oldřich (UZFG-Y)
Röslein, Jan (UZFG-Y)
Kotusz, J. (PL)
Pačes, Jan (UZFG-Y)
Pekárik, L. (SK)
Petrtýl, Miloslav (UZFG-Y)
Halačka, Karel (UBO-W) RID, SAI
Štefková Kašparová, Eva (UZFG-Y)
Mendel, Jan (UBO-W) RID, SAI, SAI, ORCID
Boron, A. (PL)
Juchno, D. (PL)
Leska, A. (PL)
Jablonska, O. (PL)
Beneš, V. (DE)
Šídová, Monika (BTO-N) RID
Janko, Karel (UZFG-Y) RID, ORCIDSource Title Molecular Biology and Evolution. - : Oxford University Press - ISSN 0737-4038
Roč. 36, č. 9 (2019), s. 1902-1920Number of pages 19 s. Publication form Online - E Language eng - English Country US - United States Keywords asexuality ; polyploidy ; hybridization ; tissue-specific gene expression ; expression-level dominance ; cis-trans-regulation Subject RIV EB - Genetics ; Molecular Biology OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology Subject RIV - cooperation Institute of Vertebrate Biology - Zoology
Institute of Biotechnology - Genetics ; Molecular BiologyR&D Projects EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA13-12580S GA ČR - Czech Science Foundation (CSF) GA17-09807S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 ; UBO-W - RVO:68081766 ; BTO-N - RVO:86652036 UT WOS 000493043800005 EID SCOPUS 85071001071 DOI 10.1093/molbev/msz114 Annotation Hybridization and polyploidization are important evolutionary processes whose impacts range from the alteration of gene expression and phenotypic variation to the triggering of asexual reproduction. We investigated fishes of the Cobitis taenia-elongatoides hybrid complex, which allowed us to disentangle the direct effects of both processes, due to the co-occurrence of parental species with their diploid and triploid hybrids. Employing morphological, ecological, and RNAseq approaches, we investigated the molecular determinants of hybrid and polyploid forms.
In contrast with other studies, hybridization and polyploidy induced relatively very little transgressivity. Instead, Cobitis hybrids appeared intermediate with a clear effect of genomic dosing when triploids expressed higher similarity to the parent contributing two genome sets. This dosage effect was symmetric in the germline (oocyte gene expression), interestingly though, we observed an overall bias toward C. taenia in somatic tissues and traits. At the level of individual genes, expression-level dominance vastly prevailed over additivity or transgressivity. Also, trans-regulation of gene expression was less efficient in diploid hybrids than in triploids, where the expression modulation of homoeologs derived from the haploid parent was stronger than those derived from the diploid parent.
Our findings suggest that the apparent intermediacy of hybrid phenotypes results from the combination of individual genes with dominant expression rather than from simple additivity. The efficiency of cross-talk between trans-regulatory elements further appears dosage dependent. Important effects of polyploidization may thus stem from changes in relative concentrations of trans-regulatory elements and their binding sites between hybridizing genomes. Links between gene regulation and asexuality are discussed.Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2020 Electronic address https://academic.oup.com/mbe/article/36/9/1902/5488194
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