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Interaction pathways and structure-chemical transformations of alginate gels in physiological environments

SYSNO ASEP	0510805
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Interaction pathways and structure-chemical transformations of alginate gels in physiological environments
Author(s)	Urbanová, Martina (UMCH-V)_{RID, ORCID} Pavelková, M. (CZ) Czernek, Jiří (UMCH-V)_RID Kubová, K. (CZ) Vysloužil, J. (CZ) Pechová, A. (CZ) Molinková, D. (CZ) Vysloužil, J. (CZ) Vetchý, D. (CZ) Brus, Jiří (UMCH-V)_{RID, ORCID}
Source Title	Biomacromolecules. - : American Chemical Society - ISSN 1525-7797 Roč. 20, č. 11 (2019), s. 4158-4170
Number of pages	13 s.
Language	eng - English
Country	US - United States
Keywords	alginate gels ; molecular-level interactions ; physiological conditions
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
R&D Projects	LO1507 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTAUSA18011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	UMCH-V - RVO:61389013
UT WOS	000496343800012
EID SCOPUS	85074441891
DOI	10.1021/acs.biomac.9b01052
Annotation	The remarkably diverse affinity of alginate (ALG) macromolecules for polyvalent metal ions makes cross-linked alginate gels an outstanding biomaterial. Surprisingly, however, very little is known about their interactions and structural transformations in physiological environments. To bridge this gap, we prepared a set of ALG gels cross-linked by various ions and monitored their structural changes at different media simulating gastric and intestinal fluids and cellular environments. For these studies, we used multinuclear solid-state NMR (ss-NMR) spectroscopy, which revealed a range of competitive ion-exchange and interconversion reactions, the rate of which strongly depended on the nature of the cross-linking metal ions. Depending on the environment, ALG chains adopted different forms, such as acidic (hydro)gels stabilized by strong hydrogen bonds, and/or weakly cross-linked Na/H-gels. Simultaneously, the exchanged polyvalent ions extensively interacted with the environment even forming in some cases insoluble phosphate microdomains directly deposited in the ALG bead matrix. The extent of the transformations and incorporation of secondary phases into the alginate beads followed the size and electronegativity of the cross-linking ions. Overall, the applied combination of various macroscopic and biological tests with multinuclear ss-NMR revealed a complex pathway of alginate beads transformations in physiological environments.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2020
Electronic address	https://pubs.acs.org/doi/10.1021/acs.biomac.9b01052

Number of the records: 1