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Toxicity of graphene oxide against algae and cyanobacteria: Nanoblade-morphology-induced mechanical injury and self protection mechanism
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SYSNO ASEP 0510450 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Toxicity of graphene oxide against algae and cyanobacteria: Nanoblade-morphology-induced mechanical injury and self protection mechanism Author(s) Malina, Tomáš (BU-J)
Maršálková, Eliška (BU-J) ORCID, SAI
Holá, K. (CZ)
Tuček, J. (CZ)
Scheibe, M. (CZ)
Zbořil, R. (CZ)
Maršálek, Blahoslav (BU-J) ORCID, SAISource Title Carbon. - : Elsevier - ISSN 0008-6223
Roč. 155, Dec 2019 (2019), s. 386-396Number of pages 11 s. Language eng - English Country US - United States Keywords toxicity mitigation ; graphene oxide ; algae Subject RIV EH - Ecology, Behaviour OECD category Environmental sciences (social aspects to be 5.7) R&D Projects DG16P02M041 GA MK - Ministry of Culture (MK) Method of publishing Limited access UT WOS 000491864400045 EID SCOPUS 85071864304 DOI https://doi.org/10.1016/j.carbon.2019.08.086 Annotation Graphene oxide (GO) is the most extensively studied two-dimensional material and has many potential applications in biomedicine, biotechnologies, and environmental technologies. However, its toxicological effects on aquatic organisms have not been properly investigated. Here, we compare the toxicity of differently oxidized graphene oxide systems towards the green alga Raphidocelis subcapitata and the cyanobacterium Synechococcus elongatus. The cyanobacterium exhibited higher GO sensitivity and more rapid growth inhibition than the alga, in keeping with the established antibacterial properties of GO. The toxic effects of GO included shading/aggregation of GOs and nutrient depletion, however a detailed mechanistic study revealed that GO acted against R. subcapitata via an additional, new mechanism. Remarkably, lightly oxidized GO samples induced significantly greater membrane integrity damage than more heavily oxidized GO samples. Flow cytometry and microscopy experiments revealed that lightly oxidized GO can act as a nano-blade that causes mechanical damage to algal cells, probably because of the comparatively low coverage of oxygen-bearing functionalities at the edges of such GO sheets. The degree of oxidation of GO samples thus affects their ecotoxicity. Interestingly, longer incubations activated stress-induced defense reactions involving extracellular protein and carbohydrate biosynthesis in both algae and cyanobacteria. Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2021 Electronic address https://doi.org/10.1016/j.carbon.2019.08.086
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