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Toxicity of graphene oxide against algae and cyanobacteria: Nanoblade-morphology-induced mechanical injury and self protection mechanism

SYSNO ASEP	0510450
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Toxicity of graphene oxide against algae and cyanobacteria: Nanoblade-morphology-induced mechanical injury and self protection mechanism
Author(s)	Malina, Tomáš (BU-J) Maršálková, Eliška (BU-J)_{ORCID, SAI} Holá, K. (CZ) Tuček, J. (CZ) Scheibe, M. (CZ) Zbořil, R. (CZ) Maršálek, Blahoslav (BU-J)_{ORCID, SAI}
Source Title	Carbon. - : Elsevier - ISSN 0008-6223 Roč. 155, Dec 2019 (2019), s. 386-396
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	toxicity mitigation ; graphene oxide ; algae
Subject RIV	EH - Ecology, Behaviour
OECD category	Environmental sciences (social aspects to be 5.7)
R&D Projects	DG16P02M041 GA MK - Ministry of Culture (MK)
Method of publishing	Limited access
UT WOS	000491864400045
EID SCOPUS	85071864304
DOI	10.1016/j.carbon.2019.08.086
Annotation	Graphene oxide (GO) is the most extensively studied two-dimensional material and has many potential applications in biomedicine, biotechnologies, and environmental technologies. However, its toxicological effects on aquatic organisms have not been properly investigated. Here, we compare the toxicity of differently oxidized graphene oxide systems towards the green alga Raphidocelis subcapitata and the cyanobacterium Synechococcus elongatus. The cyanobacterium exhibited higher GO sensitivity and more rapid growth inhibition than the alga, in keeping with the established antibacterial properties of GO. The toxic effects of GO included shading/aggregation of GOs and nutrient depletion, however a detailed mechanistic study revealed that GO acted against R. subcapitata via an additional, new mechanism. Remarkably, lightly oxidized GO samples induced significantly greater membrane integrity damage than more heavily oxidized GO samples. Flow cytometry and microscopy experiments revealed that lightly oxidized GO can act as a nano-blade that causes mechanical damage to algal cells, probably because of the comparatively low coverage of oxygen-bearing functionalities at the edges of such GO sheets. The degree of oxidation of GO samples thus affects their ecotoxicity. Interestingly, longer incubations activated stress-induced defense reactions involving extracellular protein and carbohydrate biosynthesis in both algae and cyanobacteria.
Workplace	Institute of Botany
Contact	Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8
Year of Publishing	2021
Electronic address	https://doi.org/10.1016/j.carbon.2019.08.086

Number of the records: 1